Overview

Description

  • Nature

    Recombinant
  • Source

    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Accession
    • Species

      Human
    • Molecular weight

      130 kDa including tags
    • Amino acids

      1 to 889
    • Tags

      GST tag N-Terminus

Associated products

Specifications

Our Abpromise guarantee covers the use of ab125615 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    The specific activity of ab125615 was determined to be 3 nmol/mg/min.
  • Applications

    Western blot

    Functional Studies

    SDS-PAGE

  • Form

    Liquid
  • Additional notes

    ab204879 (RPS6 peptide) can be utilized as a substrate for assessing kinase activity

     This product was previously labelled as PKN beta

     

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol, 0.88% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • pkn3
    • PKN3_HUMAN
    • PKNBETA
    • Protein kinase C related kinase 3
    • Protein kinase N3
    • Protein kinase PKN beta
    • Protein kinase PKN-beta
    • Protein-kinase C-related kinase 3
    • RP11-545E17.1
    • Serine/threonine protein kinase N3
    • Serine/threonine-protein kinase N3
    see all
  • Function

    Contributes to invasiveness in malignant prostate cancer.
  • Tissue specificity

    Expressed in prostate tumors and various cancer cell lines. Not expressed in adult tissues.
  • Sequence similarities

    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 protein kinase domain.
    Contains 3 REM (Hr1) repeats.
  • Domain

    The C1 domain does not bind the diacylglycerol (DAG).
  • Post-translational
    modifications

    Autophosphorylated.
  • Cellular localization

    Nucleus. Cytoplasm > perinuclear region. Nuclear and perinuclear Golgi region.
  • Information by UniProt

Images

  • Sample Kinase Assay showing the specific activity of ab125615 as 3 nmol/mg/min.
  • SDS-PAGE analysis of ab125615.

References

ab125615 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for contacting us.
I regret to inform you that the immunogen sequence homology between rat and human is only 22% so this antibody should not be cross reacting with rat protein. I apologize for an inconvenience.
This is the only product we can offer so I am sorry I did not check further the specificity towards non active or active form of protein.
I hope this information is nevertheless helpful to you. Please do not hesitate to contact us if you need any more advice or information.
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