Recombinant human PKR protein (ab85838)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 80% Densitometry
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: WB, Functional Studies, SDS-PAGE
Description
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Product name
Recombinant human PKR protein
See all PKR proteins and peptides -
Biological activity
The Specific activity of ab85838 was determined to be 53-59 nmol/min/mg dependent on lot.
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Purity
> 80 % Densitometry.
Affinity purified. -
Expression system
Baculovirus infected Sf9 cells -
Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Amino acids
252 to 551 -
Tags
GST tag N-Terminus
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Associated products
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Substrate reagent
Specifications
Our Abpromise guarantee covers the use of ab85838 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
Functional Studies
SDS-PAGE
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Form
Liquid -
Additional notes
ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- Double stranded RNA activated protein kinase;
- E2AK2_HUMAN
- eIF-2A protein kinase 2
see all -
Function
Following activation by double-stranded RNA in the presence of ATP, the kinase becomes autophosphorylated and can catalyze the phosphorylation of the translation initiation factor EIF2S1, which leads to an inhibition of the initiation of protein synthesis. Double-stranded RNA is generated during the course of a viral infection. -
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.
Contains 2 DRBM (double-stranded RNA-binding) domains.
Contains 1 protein kinase domain. -
Post-translational
modificationsAutophosphorylated on several Ser and Thr residues. Autophosphorylation of Thr-451 is dependent on Thr-446 and is stimulated by dsRNA binding and dimerization. Autophosphorylation apparently leads to the activation of the kinase. - Information by UniProt
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab85838 has not yet been referenced specifically in any publications.