Description

  • Product name

    Recombinant Human PLA2G1B protein
    See all PLA2G1B proteins and peptides
  • Purity

    > 95 % SDS-PAGE.

  • Expression system

    Escherichia coli
  • Accession

  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      MRGSHHHHHH GMASHMAVWQ FRKMIKCVIP GSDPFLEYNN YGCYCGLGGS GTPVDELDKC CQTHDNCYDQ AKKLDSCKFL LDNPYTHTYS YSCSGSAITC SSKNKECEAF ICNCDRNAAI CFSKAPYNKA HKNLDTKKYC QS
    • Amino acids

      23 to 148
    • Tags

      His tag N-Terminus
    • Additional sequence information

      Total 142 AA. MW: 16 kDa (calculated). N-Terminal His-tag, 16 extra AA

Specifications

Our Abpromise guarantee covers the use of ab63842 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

  • Form

    Lyophilised
  • Additional notes

    Product is NOT sterile! Please filter the product by an appropriate sterile filter before using it in cell culture.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 4.00
    Constituent: 0.41% Sodium acetate

  • Reconstitution
    Add 0.1M Acetate buffer pH4 to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10µg/ml. In higher concentrations the solubility of this antigen is limited.

General Info

  • Alternative names

    • Group IB phospholipase A2
    • Group IB secretory phospholipase A2
    • MGC119834
    • MGC119835
    • PA21B_HUMAN
    • Phosphatidylcholine 2 acylhydrolase
    • Phosphatidylcholine 2-acylhydrolase 1B
    • Phospholipase A2
    • Phospholipase A2 group IB
    • Phospholipase A2 group IB (pancreas)
    • PLA 2
    • PLA2
    • PLA2A
    • PLA2G1B
    • PPLA 2
    • PPLA2
    • sPLA2IB
    see all
  • Function

    PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.
  • Sequence similarities

    Belongs to the phospholipase A2 family.
  • Cellular localization

    Secreted.
  • Information by UniProt

Images

  • 12% SDS-PAGE separation of ab63842. Molecular Weight: ~16 kDa.

    Lane 1. Molecular weight marker
    Lane 2. reduced and heated sample, 5μg/lane
    Lane 3. non-reduced and non-heated sample, 5μg/lane

References

ab63842 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for providing that information and sorry for the delay in getting back to you.
The information provided has been very useful and I will look into whether these antibodies would be likely to detect the protein and what may be contributing to the problems observed.
In order to understand the problems encountered further, would you be able to give me an outline of the dilutions of the antibodies and proteins used, as well as any blocking steps and explain a little about how a positive signal is deduced (i.e. is a secondary antibody with a conjugate used)?
Also, which antibody combinations were used and achieved a good signal with the positive control and negative control canine samples?
I am sorry but I will be away from the office on Friday but I will get back to you with further information on Monday.
I look forward to receiving your reply.

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