Description

  • Product name

    Recombinant Human PLA2G5 protein
    See all PLA2G5 proteins and peptides
  • Purity

    > 95 % SDS-PAGE.

  • Expression system

    Escherichia coli
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      MRGSHHHHHH GMASHMGLLD LKSMIEKVTG KNALTNYGFY GCYCGWGGRG TPKDGTDWCC WAHDHCYGRL EEKGCNIRTQ SYKYRFAWGV VTCEPGPFCH VNLCACDRKL VYCLKRNLRS YNPQYQYFPN ILCS
    • Amino acids

      21 to 138

Specifications

Our Abpromise guarantee covers the use of ab63846 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

  • Form

    Lyophilised
  • Additional notes

    Product is NOT sterile! Please filter the product by an appropriate sterile filter before using it in cell culture.

     This product was previously labelled as Secretory phospholipase A2 Type V

     

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 4.00
    Constituent: 0.41% Sodium acetate

  • Reconstitution
    Add 0.1M Acetate buffer pH4 to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10µg/ml. In higher concentrations the solubility of this antigen is limited.

General Info

  • Alternative names

    • Ca2+ dependent phospholipase A2
    • Calcium dependent phospholipase A2
    • Calcium-dependent phospholipase A2
    • DKFZp686C2294
    • FRFB
    • Group V phospholipase A2
    • GV PLA2
    • gVPLA2
    • hVPLA(2)
    • MGC46205
    • OTTHUMP00000044655
    • PA2G5_HUMAN
    • Phosphatidylcholine 2 acylhydrolase
    • Phosphatidylcholine 2-acylhydrolase 5
    • Phospholipase A2 group V
    • PLA2 10
    • PLA2 G5
    • PLA2-10
    • Pla2g5
    • sPLA2 Type V
    see all
  • Function

    PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. This isozyme hydrolyzes more efficiently L-alpha-1-palmitoyl-2-oleoyl phosphatidylcholine than L-alpha-1-palmitoyl-2-arachidonyl phosphatidylcholine, L-alpha-1-palmitoyl-2-arachidonyl phosphatidylethanolamine, or L-alpha-1-stearoyl-2-arachidonyl phosphatidylinositol. May be involved in the production of lung surfactant, the remodeling or regulation of cardiac muscle.
  • Tissue specificity

    Heart, placenta and less abundantly, in lung.
  • Sequence similarities

    Belongs to the phospholipase A2 family.
  • Post-translational
    modifications

    This enzyme lacks one of the seven disulfide bonds found in similar PA2 proteins.
  • Cellular localization

    Secreted.
  • Information by UniProt

Images

  • 12% SDS-PAGE separation of ab63846. Molecular Weight: ~15.5 kDa.

    Lane 1. Molecular weight marker
    Lane 2. reduced and heated sample, 5μg/lane
    Lane 3. non-reduced and non-heated sample, 5μg/lane

References

This product has been referenced in:

  • López-Sagaseta J  et al. sPLA2-V inhibits EPCR anticoagulant and antiapoptotic properties by accommodating lysophosphatidylcholine or PAF in the hydrophobic groove. Blood 119:2914-21 (2012). Human . Read more (PubMed: 22167755) »
See 1 Publication for this product

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