Overview

  • Product name

    Recombinant Human PLCZ1 protein
  • Protein length

    Protein fragment

Description

  • Nature

    Recombinant
  • Source

    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species

      Human
    • Molecular weight

      29 kDa
    • Amino acids

      322 to 572
    • Tags

      His-DHFR tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab127842 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Form

    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20ºC.

    Constituents: 0.32% Tris HCl, 0.58% Sodium chloride

  • Reconstitution
    Reconstitute with water to desired concentration.

General Info

  • Alternative names

    • 1 phosphatidylinositol 4,5 bisphosphate phosphodiesterase zeta 1
    • 1-phosphatidylinositol-4
    • 5-bisphosphate phosphodiesterase zeta-1
    • MGC149685
    • NYD SP27
    • Phosphoinositide phospholipase C zeta 1
    • Phosphoinositide phospholipase C-zeta-1
    • Phospholipase C zeta 1
    • Phospholipase C-zeta-1
    • PI phospholipase C zeta 1
    • PLC zeta 1
    • PLC-zeta-1
    • PLCZ1
    • PLCZ1_HUMAN
    • PLCzeta
    • Testis development protein NYD SP27
    • Testis development related NYD SP27
    • Testis-development protein NYD-SP27
    see all
  • Function

    The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. In vitro, hydrolyzes PtdIns(4,5)P2 in a Ca(2+)-dependent manner. Triggers intracellular Ca(2+) oscillations in oocytes solely during M phase and is involved in inducing oocyte activation and initiating embryonic development up to the blastocyst stage. Is therefore a strong candidate for the egg-activating soluble sperm factor that is transferred from the sperm into the egg cytoplasm following gamete membrane fusion. May exert an inhibitory effect on phospholipase-C-coupled processes that depend on calcium ions and protein kinase C, including CFTR trafficking and function.
  • Tissue specificity

    Expressed specifically in testis. Weakly expressed in pancreatic-duct cells. Up-regulated in pancreatic-duct cells from patients with cystic fibrosis.
  • Sequence similarities

    Contains 1 C2 domain.
    Contains 1 EF-hand domain.
    Contains 1 PI-PLC X-box domain.
    Contains 1 PI-PLC Y-box domain.
  • Domain

    The EF-hand and C2 domains are essential for triggering Ca(2+) oscillating activity and the regulation of PLCZ1 enzyme activity.
    The X-Y linker region between PI-PLC X-box and Y-box domains may be a target for proteolysis and may play an important regulatory role during fertilization.
  • Cellular localization

    Nucleus. Cytoplasm > perinuclear region. Exhibits alternative cytoplasmic/nuclear localization during development. Translocates from the pronucleus into cytoplasm upon nuclear envelope breakdown for mitosis and localizes again to the pronucleus at interphase following meiosis and mitosis.
  • Information by UniProt

References

ab127842 has not yet been referenced specifically in any publications.

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