The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Measured in a cell proliferation assay using MDA - MB – 231 Human breast cancer cells.
The ED50 for this effect is typically 1 - 10 µg/ml.
< 1.000 Eu/µg
>95% by SDS-PAGE .
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at 4°C prior to reconstitution. Store at -20°C or -80°C.
Information available upon request.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
It is recommended to reconstitute in 20 µl sterile deionized water to a final concentration of 1 mg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage. After reconstitution store under sterile conditions for 1 month at 4°C - 8°C or 3 months at -20°C to -80°C.
Placenta growth factor
Placental growth factor
Placental growth factor, vascular endothelial growth factor related protein
Growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Isoform PlGF-2 binds NRP1/neuropilin-1 and NRP2/neuropilin-2 in a heparin-dependent manner.
While the three isoforms are present in most placental tissues, PlGF-2 is specific to early (8 week) placenta and only PlGF-1 is found in the colon and mammary carcinomas.
Belongs to the PDGF/VEGF growth factor family.
Isoform PlGF-2 contains a basic insert which acts as a cell retention signal.
Secreted. The three isoforms are secreted but PlGF-2 appears to remain cell attached unless released by heparin.