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  1. Link

    recombinant-human-plk1-protein-ab51426.pdf

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Cell Biology Cell Cycle Kinases/Phosphatases Other
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Recombinant human PLK1 protein (ab51426)

  • Datasheet
  • SDS
Submit a review Submit a question References (1)

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Functional Studies - Recombinant human PLK1 protein (ab51426)
  • SDS-PAGE - Recombinant human PLK1 protein (ab51426)
  • Functional Studies - Recombinant human PLK1 protein (ab51426)
  • SDS-PAGE - Recombinant human PLK1 protein (ab51426)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Recombinant human PLK1 protein (ab51426)

Key features and details

  • Expression system: Baculovirus infected Sf9 cells
  • Purity: > 70% Affinity purified
  • Active: Yes
  • Tags: His tag N-Terminus
  • Suitable for: WB, Functional Studies, SDS-PAGE, IHC-P

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Description

  • Product name

    Recombinant human PLK1 protein
    See all PLK1 proteins and peptides
  • Biological activity

    Specific activity 17nmol/min/mg.

  • Purity

    > 70 % Affinity purified.
    Purified by affinity chromatography
  • Expression system

    Baculovirus infected Sf9 cells
  • Accession

    P53350
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      MSAAVTAGKL ARAPADPGKA GVPGVAAPGA PAAAPPAKEI PEVLVDPRSR RRYVRGRFLG KGGFAKCFEI SDADTKEVFA GKIVPKSLLL KPHQREKMSM EISIHRSLAH QHVVGFHGFF EDNDFVFVVL ELCRRRSLLE LHKRRKALTE PEARYYLRQI VLGCQYLHRN RVIHRDLKLG NLFLNEDLEV KIGDFGLATK VEYDGERKKT LCGTPNYIAP EVLSKKGHSF EVDVWSIGCI MYTLLVGKPP FETSCLKETY LRIKKNEYSI PKHINPVAAS LIQKMLQTDP TARPTINELL NDEFFTSGYI PARLPITCLT IPPRFSIAPS SLDPSNRKPL TVLNKGLENP LPERPREKEE PVVRETGEVV DCHLSDMLQQ LHSVNASKPS ERGLVRQEEA EDPACIPIFW VSKWVDYSDK YGLGYQLCDN SVGVLFNDST RLILYNDGDS LQYIERDGTE SYLTVSSHPN SLMKKITLLK YFRNYMSEHL LKAGANITPR EGDELARLPY LRTWFRTRSA IILHLSNGSV QINFFQDHTK LILCPLMAAV TYIDEKRDFR TYRLSLLEEY GCCKELASRL RYARTMVDKL LSSRSASNRL KAS
    • Predicted molecular weight

      70 kDa
    • Amino acids

      1 to 603
    • Tags

      His tag N-Terminus

Associated products

  • Related Products

    • Anti-PLK1 antibody [35-206] (ab17056)
    • Anti-PLK1 antibody [36-298] (ab17057)

Specifications

Our Abpromise guarantee covers the use of ab51426 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    Functional Studies

    SDS-PAGE

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

  • Form

    Liquid
  • Additional notes

    ab91090 (Cow Casein full length protein) can be utilized as a substrate for assessing Kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.00
    Constituents: 0.00174% PMSF, 0.82% Sodium phosphate, 0.00308% DTT, 25% Glycerol (glycerin, glycerine), 1.74% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • Cell cycle regulated protein kinase
    • PLK
    • PLK 1
    • PLK-1
    • plk1
    • PLK1_HUMAN
    • Polo like kinase 1
    • Polo-like kinase 1
    • Serine/threonine protein kinase 13
    • Serine/threonine protein kinase PLK1
    • Serine/threonine-protein kinase 13
    • Serine/threonine-protein kinase PLK1
    • STPK 13
    • STPK13
    see all
  • Function

    Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis. Required for recovery after DNA damage checkpoint and entry into mitosis. Required for kinetochore localization of BUB1B. Phosphorylates SGOL1. Required for spindle pole localization of isoform 3 of SGOL1 and plays a role in regulating its centriole cohesion function. Phosphorylates BORA, and thereby promotes the degradation of BORA. Contributes to the regulation of AURKA function. Regulates TP53 stability through phosphorylation of TOPORS.
  • Tissue specificity

    Placenta and colon.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.
    Contains 2 POLO box domains.
    Contains 1 protein kinase domain.
  • Developmental stage

    Accumulates to a maximum during the G2 and M phases, declines to a nearly undetectable level following mitosis and throughout G1 phase, and then begins to accumulate again during S phase.
  • Post-translational
    modifications

    Catalytic activity is enhanced by phosphorylation of Thr-210. Phosphorylation at Thr-210 is first detected on centrosomes in the G2 phase of the cell cycle, peaks in prometaphase and gradually disappears from centrosomes during anaphase.
    Autophosphorylation and phosphorylation of Ser-137 may not be significant for the activation of PLK1 during mitosis, but may enhance catalytic activity during recovery after DNA damage checkpoint.
    Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) in anaphase and following DNA damage, leading to its degradation by the proteasome. Ubiquitination is mediated via its interaction with FZR1/CDH1. Ubiquitination and subsequent degradation prevents entry into mitosis and is essential to maintain an efficient G2 DNA damage checkpoint.
  • Cellular localization

    Nucleus. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > centrosome. During early stages of mitosis, the phosphorylated form is detected on centrosomes and kinetochores. Localizes to the outer kinetochore. Presence of SGOL1 and interaction with the phosphorylated form of BUB1 is required for the kinetochore localization.
  • Target information above from: UniProt accession P53350 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

Images

  • Functional Studies - Recombinant human PLK1 protein (ab51426)
    Functional Studies - Recombinant human PLK1 protein (ab51426)
    The specific activity of PLK1 (ab51426) was determined to be 16.5 nmol/min/mg as per activity assay protocol
  • SDS-PAGE - Recombinant human PLK1 protein (ab51426)
    SDS-PAGE - Recombinant human PLK1 protein (ab51426)
    SDS PAGE analysis of ab51426
  • Functional Studies - Recombinant human PLK1 protein (ab51426)
    Functional Studies - Recombinant human PLK1 protein (ab51426)
    Kinase activity assay. Specific activity 17 nmol/min/mg.
  • SDS-PAGE - Recombinant human PLK1 protein (ab51426)
    SDS-PAGE - Recombinant human PLK1 protein (ab51426)
    Recombiant human PLK1 protein (His tagged). Molecular weight 70 kDa.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Recombinant human PLK1 protein (ab51426)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Recombinant human PLK1 protein (ab51426)

    Immunohistochemistry of Human Lymphoma tissue staining PLK1 with ab51426 at 1/40. Control is treated with fusion protein. 

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (1)

Publishing research using ab51426? Please let us know so that we can cite the reference in this datasheet.

ab51426 has been referenced in 1 publication.

  • Mao Y  et al. Targeting TEAD/YAP-transcription-dependent necrosis, TRIAD, ameliorates Huntington's disease pathology. Hum Mol Genet 25:4749-4770 (2016). PubMed: 28171658

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