Overview

Description

  • Nature
    Recombinant
  • Source
    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Species
      Human
    • Sequence
      SGR TYLKGRLLGK GGFARCYEAT DTETGSAYAV KVIPQSRVAK PHQREKILNE IELHRDLQHR HIVRFSHHFE DADNIYIFLE LCSRKSLAHI WKARHTLLEP EVRYYLRQIL SGLKYLHQRG ILHRDLKLGN FFITENMELK VGDFGLAARL EPPEQRKKTI CGTPNYVAPE VLLRQGHGPE ADVWSLGCVM YTLLCGSPPF ETADLKETYR CIKQVHYTLP ASLSLPARQL LAAILRASPR DRPSIDQILR HDFFTKGYTP DRLPISSCVT VPDLTPPNP
    • Amino acids
      58 to 339

Specifications

Our Abpromise guarantee covers the use of ab42603 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Activity: 500 U/mg. One unit is defined as the amount of enzyme that will phosphorylate 1 nmol of casein substrate per minute at pH 7.4 and 30C. Assay buffer: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 uM Na-orthovanadate, 0.5 mM ATP.
  • Applications

    Inhibition Assay

  • Form
    Liquid
  • Additional notes
    Expressed in a Bacuovirus infected Sf9 expression system. Expected molecular weight of 36.7 kDa.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.0462% DTT, 0.395% Tris HCl, 0.05% Tween, 50% Glycerol, 0.58% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • CNK
    • Cytokine Inducible Kinase
    • Cytokine-inducible serine/threonine-protein kinase
    • FGF inducible kinase
    • FGF-inducible kinase
    • FNK
    • PLK-3
    • Plk3
    • PLK3_HUMAN
    • Polo Like Kinase 3
    • Polo-like kinase 3
    • PRK
    • Proliferation Related Kinase
    • Proliferation-related kinase
    • Serine/threonine-protein kinase PLK3
    see all
  • Function
    Serine/threonine protein kinase involved in regulating M phase functions during the cell cycle. May also be part of the signaling network controlling cellular adhesion. In vitro, is able to phosphorylate CDC25C and casein.
  • Tissue specificity
    Transcripts are highly detected in placenta, lung, followed by skeletal muscle, heart, pancreas, ovaries and kidney and weakly detected in liver and brain. May have a short half-live. In cells of hematopoietic origin, strongly and exclusively detected in terminally differentiated macrophages. Transcript expression appears to be down-regulated in primary lung tumor.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.
    Contains 2 POLO box domains.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylated as cells enter mitosis and dephosphorylated as cells exit mitosis.
  • Cellular localization
    Membrane.
  • Information by UniProt

References

ab42603 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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