Recombinant Human Podoplanin / gp36 protein (ab87357)

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SDS-PAGE - Recombinant Human Podoplanin / gp36 protein (ab87357)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 95% SDS-PAGE
    • Suitable for: SDS-PAGE

    Description

    • Product name

      Recombinant Human Podoplanin / gp36 protein
      See all Podoplanin / gp36 proteins and peptides

    • Purity

      > 95 % SDS-PAGE.
      ab87357 is purified using conventional chromatography techniques.

    • Expression system

      Escherichia coli

    • Protein length

      Protein fragment

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        MGSSHHHHHH SSGLVPRGSH MASTGQPEDD TETTGLEGGV AMPGAEDDVV TPGTSEDRYK SGLTTLVATS VNSVTGIRIE DLPTSESTVH AQEQSPSATA SNVATSHSTE KVDGDTQTTV EKDGLSTVTL

      • Amino acids

        99 to 207

    Specifications

    Our Abpromise guarantee covers the use of ab87357 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

      pH: 7.50
      Constituents: 0.242% Tris, 0.58% Sodium chloride

    General Info

    • Alternative names

      • Aggrus
      • Glycoprotein 36 KD
      • Glycoprotein 36
      • gp 36
      • GP 38
      • GP 40
      • gp36
      • GP38
      • GP40
      • HT1A 1
      • HT1A1
      • hT1alpha 1
      • hT1alpha 2
      • hT1alpha1
      • hT1alpha2
      • Lung type I cell membrane associated glycoprotein
      • Lung type I cell membrane associated glycoprotein isoform a
      • Lung type I cell membrane associated glycoprotein T1A 2
      • OTS 8
      • OTS8
      • OTTHUMP00000009640
      • OTTHUMP00000044504
      • PA2.26
      • PA2.26 antigen
      • Pdpn
      • PDPN_HUMAN
      • Podoplanin
      • PSEC0003
      • PSEC0025
      • T1 alpha
      • T1 ALPHA GENE
      • T1-alpha
      • T1A
      • T1A 2
      • TI1A
      • TIA 2
      • TIA2
      see all

    • Function

      May be involved in cell migration and/or actin cytoskeleton organization. When expressed in keratinocytes, induces changes in cell morphology with transfected cells showing an elongated shape, numerous membrane protrusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion. Required for normal lung cell proliferation and alveolus formation at birth. Induces platelet aggregation. Does not have any effect on folic acid or amino acid transport. Does not function as a water channel or as a regulator of aquaporin-type water channels.

    • Tissue specificity

      Highly expressed in placenta, lung, skeletal muscle and brain. Weakly expressed in brain, kidney and liver. In placenta, expressed on the apical plasma membrane of endothelium. In lung, expressed in alveolar epithelium. Up-regulated in colorectal tumors and expressed in 25% of early oral squamous cell carcinomas.

    • Sequence similarities

      Belongs to the podoplanin family.

    • Post-translational
      modifications

      Extensively O-glycosylated. Contains sialic acid residues. O-glycosylation is necessary for platelet aggregation activity.
      The N-terminus is blocked.

    • Cellular localization

      Membrane. Cell projection > filopodium membrane. Cell projection > lamellipodium membrane. Cell projection > microvillus membrane. Cell projection > ruffle membrane. Localized to actin-rich microvilli and plasma membrane projections such as filopodia, lamellipodia and ruffles.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human Podoplanin / gp36 protein (ab87357)
      SDS-PAGE - Recombinant Human Podoplanin / gp36 protein (ab87357)
      15% SDS-PAGE showing ab87357 (3µg).
      Note: Real molecular weight on SDS-PAGE is shifted up compared to predicted molecular weight.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab87357? Please let us know so that we can cite the reference in this datasheet.

    ab87357 has not yet been referenced specifically in any publications.

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