Recombinant Human POLD1 protein (ab132036)

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SDS-PAGE - Recombinant Human POLD1 protein (ab132036)

    Key features and details

    • Expression system: Wheat germ
    • Suitable for: WB, SDS-PAGE, ELISA

    Description

    • Product name

      Recombinant Human POLD1 protein

    • Expression system

      Wheat germ

    • Accession

      P28340

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        MDGKRRPGPGPGVPPKRARGGLWDDDDAPRPSQFEEDLALMEEMEAEHRL QEQEEEELQSVLEGVADGQVPPSAIDPRWLRPTPPALDPQTEPLIFQQLE IDHYVGPAQPVPGGPPPSHGSVPVLRAFGVTDEGFSVCCHIHGFAPYFYT PAPPGFGPEHMGDLQRELNLAISRDSRGGRELTGPAVLAVELCSRESMFG YHGHGPSPFLRITVALPRLVAPARRLLEQGIRVAGLGTPSFAPYEANVDF EIRFMVDTDIVGCNWLELPAGKYALRLKEKATQCQLEADVLWSDVVSHPP EGPWQRIAPLRVLSFDIECAGRKGIFPEPERDPVIQICSLGLRWGEPEPF LRLALTLRPCAPILGAKVQSYEKEEDLLQAWSTFIRIMDPDVITGYNIQN FDLPYLISRAQTLKVQTFPFLGRVAGLCSNIRDSSFQSKQTGRRDTKVVS MVGRVQMDMLQVLLREYKLRSYTLNAVSFHFLGEQKEDVQHSIITDLQNG NDQTRRRLAVYCLKDAYLPLRLLERLMVLVNAVEMARVTGVPLSYLLSRG QQVKVVSQLLRQAMHEGLLMPVVKSEGGEDYTGATVIEPLKGYYDVPIAT LDFSSLYPSIMMAHNLCYTTLLRPGTAQKLGLTEDQFIRTPTGDEFVKTS VRKGLLPQILENLLSARKRAKAELAKETDPLRRQVLDGRQLALKVSANSV YGFTGAQVGKLPCLEISQSVTGFGRQMIEKTKQLVESKYTVENGYSTSAK VVYGDTDSVMCRFGVSSVAEAMALGREAADWVSGHFPSPIRLEFEKVYFP YLLISKKRYAGLLFSSRPDAHDRMDCKGLEAVRRDNCPLVANLVTASLRR LLIDRDPEGAVAHAQDVISDLLCNRIDISQLVITKELTRAASDYAGKQAH VELAERMRKRDPGSAPSLGDRVPYVIISAAKGVAAYMKSEDPLFVLEHSL PIDTQYYLEQQLAKPLLRIFEPILGEGRAEAVLLRGDHTRCKTVLTGKVG GLLAFAKRRNCCIGCRTVLSHQGAVCEFCQPRESELYQKEVSHLNALEER FSRLWTQCQRCQGSLHEDVICTSRDCPIFYMRKKVRKDLEDQEQLLRRFG PPGPEAW

      • Predicted molecular weight

        150 kDa including tags

      • Amino acids

        1 to 1107

    Specifications

    Our Abpromise guarantee covers the use of ab132036 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Western blot

      SDS-PAGE

      ELISA

    • Form

      Liquid

    • Additional notes

      This product was previously labelled as DNA Polymerase delta.

       

    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 8.00
      Constituents: 0.31% Glutathione, 0.79% Tris HCl

    General Info

    • Alternative names

      • Polymerase (DNA directed) delta 1 catalytic subunit
      • CDC2
      • CDC2 homolog
      • CRCS10
      • DNA directed DNA polymerase delta 1
      • DNA directed polymerase delta 1
      • DNA pol delta 1
      • DNA polymerase delta catalytic subunit
      • DNA polymerase subunit delta p125
      • DPOD1_HUMAN
      • MDPL
      • POLD
      • POLD 1
      • POLD1
      • Polymerase (DNA directed) delta 1 catalytic subunit 125kDa
      • Polymerase (DNA) delta 1 catalytic subunit
      • Polymerase DNA directed delta 1 catalytic subunit 125kD
      • polymerase, DNA, delta
      see all

    • Function

      Possesses two enzymatic activities: DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Required with its accessory proteins (proliferating cell nuclear antigen (PCNA) and replication factor C (RFC) or activator 1) for leading strand synthesis. Also involved in completing Okazaki fragments initiated by the DNA polymerase alpha/primase complex.

    • Sequence similarities

      Belongs to the DNA polymerase type-B family.

    • Cellular localization

      Nucleus.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human POLD1 protein (ab132036)
      SDS-PAGE - Recombinant Human POLD1 protein (ab132036)
      12.5% SDS-PAGE stained with Coomassie Blue showing ab132036 at approximately 150 kDa.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab132036? Please let us know so that we can cite the reference in this datasheet.

    ab132036 has not yet been referenced specifically in any publications.

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