Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 81% SDS-PAGE
- Tags: His tag N-Terminus, DDDDK tag N-Terminus
- Suitable for: SDS-PAGE
Product nameRecombinant Human POLG protein
See all POLG proteins and peptides
Purity>= 81 % SDS-PAGE.
Expression systemBaculovirus infected Sf9 cells
Protein lengthFull length protein
SequenceMHHHHHHDYKDDDDKSRLLWRKVAGATVGPGPVPAPGRWVSSSVPASDPS DGQRR RQQQQQQQQQQQQQPQQPQVLSSEGGQLRHNPLDIQMLSRGLH EQIFGQGGEMP GEAAVRRSVEHLQKHGLWGQPAVPLPDVELRLPPLYG DNLDQHFRLLAQKQSLPYL EAANLLLQAQLPPKPPAWAWAEGWTRYGP EGEAVPVAIPEERALVFDVEVCLAEGT CPTLAVAISPSAWYSWCSQRL VEERYSWTSQLSPADLIPLEVPTGASSPTQRDWQEQ LVVGHNVSFDRA HIREQYLIQGSRMRFLDTMSMHMAISGLSSFQRSLWIAAKQGKHK VQP PTKQGQKSQRKARRGPAISSWDWLDISSVNSLAEVHRLYVGGPPLEKEPR ELFV KGTMKDIRENFQDLMQYCAQDVWATHEVFQQQLPLFLERCPHPV TLAGMLEMGVSY LPVNQNWERYLAEAQGTYEELQREMKKSLMDLANDA CQLLSGERYKEDPWLWDLE WDLQEFKQKKAKKVKKEPATASKLPIEGA GAPGDPMDQEDLGPCSEEEEFQQDVM ARACLQKLKGTTELLPKRPQHL PGHPGWYRKLCPRLDDPAWTPGPSLLSLQMRVTP KLMALTWDGFPLHY SERHGWGYLVPGRRDNLAKLPTGTTLESAGVVCPYRAIESLYR KHCLE QGKQQLMPQEAGLAEEFLLTDNSAIWQTVEELDYLEVEAEAKMENLRAAV P GQPLALTARGGPKDTQPSYHHGNGPYNDVDIPGCWFFKLPHKDGNSC NVGSPFAKD FLPKMEDGTLQAGPGGASGPRALEINKMISFWRNAHKRI SSQMVVWLPRSALPRAVI RHPDYDEEGLYGAILPQVVTAGTITRRAVE PTWLTASNARPDRVGSELKAMVQAPPG YTLVGADVDSQELWIAAVLGD AHFAGMHGCTAFGWMTLQGRKSRGTDLHSKTATTV GISREHAKIFNYG RIYGAGQPFAERLLMQFNHRLTQQEAAEKAQQMYAATKGLRWY RLSDE GEWLVRELNLPVDRTEGGWISLQDLRKVQRETARKSQWKKWEVVAERAWK GGTESEMFNKLESIATSDIPRTPVLGCCISRALEPSAVQEEFMTSRVN WVVQSSAVDY LHLMLVAMKWLFEEFAIDGRFCISIHDEVRYLVREEDR YRAALALQITNLLTRCMFAYK LGLNDLPQSVAFFSAVDIDRCLRKEVT MDCKTPSNPTGMERRYGIPQGEALDIYQIIEL TKGSLEKRSQPGP
Predicted molecular weight141 kDa including tags
Amino acids2 to 1239
TagsHis tag N-Terminus , DDDDK tag N-Terminus
Additional sequence information(NM_002693)
- Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab1162)
- Anti-POLG antibody [EPR7296] (ab128899)
- Anti-POLG antibody (ab154310)
- Anti-6X His tag® antibody [HIS.H8] (ab18184)
- Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab21536)
- Anti-6X His tag® antibody [4D11] (ab5000)
- Anti-6X His tag® antibody (ab9108)
- Anti-POLG antibody (ab97661)
Our Abpromise guarantee covers the use of ab196066 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
This product was previously labelled as DNA Polymerase gamma
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.04% Tween, 20% Glycerol (glycerin, glycerine)
also contains 80 µg/ml DDDDK peptide
- DNA directed DNA polymerase gamma
- DNA polymerase subunit gamma 1
- DNA polymerase subunit gamma-1
FunctionInvolved in the replication of mitochondrial DNA.
Involvement in diseaseDefects in POLG are the cause of progressive external ophthalmoplegia with mitochondrial DNA deletions autosomal dominant type 1 (PEOA1) [MIM:157640]. Progressive external ophthalmoplegia is characterized by progressive weakness of ocular muscles and levator muscle of the upper eyelid. In a minority of cases, it is associated with skeletal myopathy, which predominantly involves axial or proximal muscles and which causes abnormal fatigability and even permanent muscle weakness. Ragged-red fibers and atrophy are found on muscle biopsy. A large proportion of chronic ophthalmoplegias are associated with other symptoms, leading to a multisystemic pattern of this disease. Additional symptoms are variable, and may include cataracts, hearing loss, sensory axonal neuropathy, ataxia, depression, hypogonadism, and parkinsonism.
Defects in POLG are a cause of progressive external ophthalmoplegia with mitochondrial DNA deletions autosomal recessive (PEOB) [MIM:258450]. PEOB is a severe form of progressive external ophthalmoplegia. It is clinically more heterogeneous than the autosomal dominant forms. Can be more severe.
Defects in POLG are a cause of sensory ataxic neuropathy dysarthria and ophthalmoparesis (SANDO) [MIM:607459]. SANDO is a clinically heterogeneous systemic disorder with variable features resulting from mitochondrial dysfunction. It shares phenotypic characteristics with autosomal recessive progressive external ophthalmoplegia and mitochondrial neurogastrointestinal encephalopathy syndrome. The clinical triad of symptoms consists of sensory ataxic, neuropathy, dysarthria, and ophthalmoparesis.
Defects in POLG are a cause of Alpers-Huttenlocher syndrome (AHS) [MIM:203700]; also called Alpers diffuse degeneration of cerebral gray matter with hepatic cirrhosis. AHS is an autosomal recessive hepatocerebral syndrome. The typical course of AHS includes severe developmental delay, intractable seizures, liver failure, and death in childhood. Refractory seizures, cortical blindness, progressive liver dysfunction, and acute liver failure after exposure to valproic acid are considered diagnostic features. The neuropathological hallmarks of AHS are neuronal loss, spongiform degeneration, and astrocytosis of the visual cortex. Liver biopsy results show steatosis, often progressing to cirrhosis.
Defects in POLG are a cause of mitochondrial neurogastrointestinal encephalopathy syndrome (MNGIE) [MIM:603041]; also known as myoneurogastrointestinal encephalomyopathy. MNGIE is an autosomal recessive disease associated with multiple deletions of skeletal muscle mitochondrial DNA (MtDNA). It is clinically characterized by onset between the second and fifth decades of life, ptosis, progressive external ophthalmoplegia, gastrointestinal dysmotility (often pseudoobstruction), diffuse leukoencephalopathy, thin body habitus, peripheral neuropathy, and myopathy.
Defects in POLG are a cause of Leigh syndrome (LS) [MIM:256000]. LS is a severe neurological disorder characterized by bilaterally symmetrical necrotic lesions in subcortical brain regions.
Sequence similaritiesBelongs to the DNA polymerase type-A family.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab196066 has not yet been referenced specifically in any publications.