Bioactive grade

Recombinant human Poliovirus Receptor/PVR protein (Fc Chimera Active) (ab220469)

Description

  • Product name

    Recombinant human Poliovirus Receptor/PVR protein (Fc Chimera Active)
    See all Poliovirus Receptor/PVR proteins and peptides
  • Biological activity

    Measured by its binding ability in a functional ELISA. Immobilized Human TIGIT, Fc Tag at 2 μg/ml (100 μl/well) can bind ab220469 with a linear range of 0.98-7.81 ng/ml.

    Measured by its binding ability in a functional ELISA. Immobilized Human DNAM-1, Fc Tag at 2 μg/ml (100 μl/well) can bind ab220469 with a linear range of 0.98-15.63 ng/ml.

  • Purity

    > 95 % SDS-PAGE.

  • Endotoxin level

    < 0.100 Eu/µg
  • Expression system

    HEK 293 cells
  • Accession

  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      WPPPGTGDVVVQAPTQVPGFLGDSVTLPCYLQVPNMEVTHVSQLTWARHG ESGSMAVFHQTQGPSYSESKRLEFVAARLGAELRNASLRMFGLRVEDEGN YTCLFVTFPQGSRSVDIWLRVLAKPQNTAEVQKVQLTGEPVPMARCVSTG GRPPAQITWHSDLGGMPNTSQVPGFLSGTVTVTSLWILVPSSQVDGKNVT CKVEHESFEKPQLLTVNLTVYYPPEVSISGYDNNWYLGQNEATLTCDARS NPEPTGYNWSTTMGPLPPFAVAQGAQLLIRPVDKPINTTLICNVTNALGA RQAELTVQVKEGPPSEHSGMSRN
    • Predicted molecular weight

      62 kDa including tags
    • Amino acids

      21 to 343
    • Additional sequence information

      Extracellular domain fused with a mouse IgG2a Fc tag at the C-terminus (NP_006496.2).

Associated products

Specifications

Our Abpromise guarantee covers the use of ab220469 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Form

    Lyophilised
  • Additional notes

     This product was previously labelled as Poliovirus Receptor

     

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 7.50
    Constituents: 0.61% Tris, 0.75% Glycine, 5% Trehalose, 0.44% L-Arginine, 0.87% Sodium chloride

    Lyophilized from 0.22 µm filtered solution.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    It is recommended to reconstitute the lyophilized product in sterile deionized water to a final concentration of 1 mg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage.

General Info

  • Alternative names

    • CD155
    • CD155 antigen
    • FLJ25946
    • HVED
    • mE4
    • NECL 5
    • Necl-5
    • Necl5
    • Nectin like 5
    • Nectin like protein 5
    • Nectin-like protein 5
    • Ortholog of mouse Tage4
    • Poliovirus receptor
    • PVR
    • PVR_HUMAN
    • PVS
    • Taa1
    • Tage 4
    • TAGE4
    see all
  • Function

    Mediates NK cell adhesion and triggers NK cell effector functions. Binds two different NK cell receptors: CD96 and CD226. These interactions accumulates at the cell-cell contact site, leading to the formation of a mature immunological synapse between NK cell and target cell. This may trigger adhesion and secretion of lytic granules and IFN-gamma and activate cytoxicity of activated NK cells. May also promote NK cell-target cell modular exchange, and PVR transfer to the NK cell. This transfer is more important in some tumor cells expressing a lot of PVR, and may trigger fratricide NK cell activation, providing tumors with a mechanism of immunoevasion. Plays a role in mediating tumor cell invasion and migration. Serves as a receptor for poliovirus attachment to target cells. May play a role in axonal transport of poliovirus, by targeting virion-PVR-containing endocytic vesicles to the microtubular network through interaction with DYNLT1. This interaction would drive the virus-containing vesicle to the axonal retrograde transport.
  • Sequence similarities

    Belongs to the nectin family.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt

Images

  • FACS analysis shows that the binding of Human CD155, mouse IgG2a Fc Tag to 293T overexpressing TIGIT was inhibited by increasing concentration of neutralizing Anti-Human TIGIT MAb. The concentration of CD155 used is 1 µg/ml. The IC50 is 0.1413 µg/ml.
  • FACS assay shows that recombinant Human CD155, mouse IgG2a Fc Tag can bind to 293T cell overexpressing human TIGIT. The concentration of CD155 is 1 µg/ml.
  • SDS-PAGE analysis of ab220469 stained overnight with Coomassie Blue.

    Lane 1: Reducing conditions.

    Lane 2: Non-reducing conditions.

     

    The protein migrates as 70-100 kDa under reducing conditions and 130-160 kDa under non-reducing conditions on SDS-PAGE gel.

     

  •  Immobilized Human TIGIT, Fc Tag at 2 μg/ml (100 μl/well) can bind ab220469 with a linear range of 0.98-7.81 ng/ml.

  • Immobilized Human DNAM-1, Fc Tag at 2 μg/ml (100 μl/well) can bind ab220469 with a linear range of 0.98-15.63 ng/ml.

References

ab220469 has not yet been referenced specifically in any publications.

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