Bioactive grade

Recombinant human Poliovirus Receptor/PVR protein (His tag) (ab216250)

Description

  • Product name

    Recombinant human Poliovirus Receptor/PVR protein (His tag)
    See all Poliovirus Receptor/PVR proteins and peptides
  • Biological activity

    ab216250 binding to TIGIT was confirmed according as follow: briefly, 4 ng of TIGIT-biotin was mixed with varying amounts of ab216250 in TIGIT assay buffer at a final volume of 10 μl and allowed to incubate for 1 hr at room temp. Nickle acceptor beads were added for 30 min, followed by Strep-conjugated acceptor beads for 30 min and reading alpha-counts.

  • Purity

    > 90 % SDS-PAGE.

  • Endotoxin level

    < 1.000 Eu/µg
  • Expression system

    HEK 293 cells
  • Accession

  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      GDVVVQAPTQVPGFLGDSVTLPCYLQVPNMEVTHVSQLTWARHGESGSMA VFHQTQGPSYSESKRLEFVAARLGAELRNASLRMFGLRVEDEGNYTCLFV TFPQGSRSVDIWLRVLAKPQNTAEVQKVQLTGEPVPMARCVSTGGRPPAQ ITWHSDLGGMPNTSQVPGFLSGTVTVTSLWILVPSSQVDGKNVTCKVEHE SFEKPQLLTVNLTVYYPPEVSISGYDNNWYLGQNEATLTCDARSNPEPTG YNWSTTMGPLPPFAVAQGAQLLIRPVDKPINTTLICNVTNALGARQAELT VQVKEGPPSEHSGISRN
    • Predicted molecular weight

      35 kDa including tags
    • Amino acids

      27 to 343
    • Tags

      His tag C-Terminus
    • Additional sequence information

      Extracellular domain of Poliovirus Receptor.

Specifications

Our Abpromise guarantee covers the use of ab216250 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Form

    Liquid
  • Additional notes

    Protein may be diluted to ≥ 100 μg/ml in PBS + glycerol and stored at -80°C.

     This product was previously labelled as Poliovirus Receptor

     

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.02% Potassium chloride, 0.64% Sodium chloride, 20% Glycerol, 0.63% Tris HCl

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • CD155
    • CD155 antigen
    • FLJ25946
    • HVED
    • mE4
    • NECL 5
    • Necl-5
    • Necl5
    • Nectin like 5
    • Nectin like protein 5
    • Nectin-like protein 5
    • Ortholog of mouse Tage4
    • Poliovirus receptor
    • PVR
    • PVR_HUMAN
    • PVS
    • Taa1
    • Tage 4
    • TAGE4
    see all
  • Function

    Mediates NK cell adhesion and triggers NK cell effector functions. Binds two different NK cell receptors: CD96 and CD226. These interactions accumulates at the cell-cell contact site, leading to the formation of a mature immunological synapse between NK cell and target cell. This may trigger adhesion and secretion of lytic granules and IFN-gamma and activate cytoxicity of activated NK cells. May also promote NK cell-target cell modular exchange, and PVR transfer to the NK cell. This transfer is more important in some tumor cells expressing a lot of PVR, and may trigger fratricide NK cell activation, providing tumors with a mechanism of immunoevasion. Plays a role in mediating tumor cell invasion and migration. Serves as a receptor for poliovirus attachment to target cells. May play a role in axonal transport of poliovirus, by targeting virion-PVR-containing endocytic vesicles to the microtubular network through interaction with DYNLT1. This interaction would drive the virus-containing vesicle to the axonal retrograde transport.
  • Sequence similarities

    Belongs to the nectin family.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt

Images

  • 4-20% SDS-PAGE using 3μg of ab216250.

  • Poliovirus Receptor/PVR protein interaction assay. Briefly, 4 ng of TIGIT-biotin was mixed with varying amounts of ab216250 in TIGIT assay buffer at a final volume of 10 μl and allowed to incubate for 1 hr at room temp. Nickle acceptor beads were added for 30 min, followed by Strep-conjugated acceptor beads for 30 min and reading alpha-counts.

References

ab216250 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab216250.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up