The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Protein concentration is above or equal to 0.05 mg/ml.
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Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 8.00 Constituents: 0.31% Glutathione, 0.79% Tris HCl
DNA directed RNA polymerase I
DNA directed RNA polymerase I 135kDa polypeptide
DNA directed RNA polymerase I subunit RPA2
DNA-directed RNA polymerase I 135 kDa polypeptide
DNA-directed RNA polymerase I subunit rpa2
Polymerase (RNA) I polypeptide B
Polymerase (RNA) I polypeptide B 128kDa
RNA polymerase I polypeptide B
RNA polymerase I subunit 2
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol I is composed of mobile elements and RPA2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft.