Description

  • Product name

    Recombinant Human POLR2F protein
  • Purity

    > 95 % SDS-PAGE.
    purified by using conventional chromatography techniques.
  • Expression system

    Escherichia coli
  • Accession

  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      MGSSHHHHHH SSGLVPRGSH MGSMSDNEDN FDGDDFDDVE EDEGLDDLEN AEEEGQENVE ILPSGERPQA NQKRITTPYM TKYERARVLG TRALQIAMCA PVMVELEGET DPLLIAMKEL KARKIPIIIR RYLPDGSYED WGVDELIITD
    • Predicted molecular weight

      17 kDa including tags
    • Amino acids

      1 to 127
    • Tags

      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab180291 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Mass Spectrometry

    SDS-PAGE

  • Mass spectrometry

    MALDI-TOF
  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8.0
    Constituents: 0.32% Tris HCl, 0.88% Sodium chloride, 0.02% DTT, 20% Glycerol

General Info

  • Alternative names

    • DNA directed RNA polymerase II 14.4 kda polypeptide
    • DNA directed RNA polymerase II polypeptide F
    • DNA directed RNA polymerases I, II, and III 14.4 kDa polypeptide
    • HRBP14.4
    • hsRPB6
    • POLRF
    • polymerase (RNA) II (DNA directed) polypeptide F
    • RPABC14.4
    • RPABC2
    • RPB14.4
    • RPB6
    see all
  • Relevance

    POLR2F belongs to the archaeal rpoK/eukaryotic RPB6 RNA polymerase subunit family and is the sixth largest subunit of RNA polymerase II. This polymerase, along with two other DNA-directed RNA polymerases, is responsible for synthesizing messenger RNA in eukaryotes. Three distinct zinc-containing RNA polymerases are found in eukaryotic nuclei: polymerase I for the ribosomal RNA precursor, polymerase II for the mRNA precursor, and polymerase III for 5S and tRNA genes. Each class of RNA polymerase is assembled from 9 to 15 different polypeptides. In yeast, this polymerase subunit, in combination with at least two other subunits, forms a structure that stabilizes the transcribing polymerase on the DNA template.
  • Cellular localization

    Nuclear

Images

  • 15% SDS-PAGE (3μg)

References

ab180291 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab180291.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up