Recombinant Human Poly-ubiquitin (linkage-specific K48) protein (ab206187)

Description

  • Product name

    Recombinant Human Poly-ubiquitin (linkage-specific K48) protein
    See all Poly-ubiquitin proteins and peptides
  • Purity

    > 95 % SDS-PAGE.

  • Expression system

    Escherichia coli
  • Accession

  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Amino acids

      1 to 76

Specifications

Our Abpromise guarantee covers the use of ab206187 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Form

    Liquid
  • Additional notes

    Ubiquitin chain products are produced by using Human wild type Ubiquitin reacting with specific E2s.

    Ubiquitin chains, especially K63-linked ones, often form soluble aggregates even storing at -80°C. If necessary, urea powder can be added into the stock solution up to 3 M, then keep the stock solution at room temperature for 30 minutes. This treatment has no effect on Ubiquitin chain structure, but breaks soluble Ubiquitin chain aggregates.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.

    Constituents: 10% Glycerol, 0.02% Beta mercaptoethanol, 0.87% Sodium chloride, 0.24% Tris

General Info

  • Alternative names

    • UBB
    • UBB_HUMAN
    • Ubiquitin
  • Function

    Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.
  • Sequence similarities

    Belongs to the ubiquitin family.
    Contains 3 ubiquitin-like domains.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt

Images

  • Coomassie-stained SDS-PAGE analysis of ab206187.
    Lane 1: 5 µg purified K48-Ub2
    Lane 2: 5 µg purified K48-Ub3
    Lane 3: 5 µg purified K48-Ub4
    Lane 4: 5 µg purified K48-Ub5
    Lane 5: 5 µg ab206187

References

ab206187 has not yet been referenced specifically in any publications.

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