Recombinant Human Poly-ubiquitin (linkage-specific K63) protein (ab206177)
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Description
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Product name
Recombinant Human Poly-ubiquitin (linkage-specific K63) protein
See all Poly-ubiquitin proteins and peptides -
Purity
> 95 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Modifications
linkage-specific K63
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Specifications
Our Abpromise guarantee covers the use of ab206177 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Liquid -
Additional notes
Ub chains, especially K63-linked ones, often form soluble aggregates even storing at -80°C. If necessary, urea powder can be added into the stock solution up to 3 M, then keep the stock solution at room temperature for 30 minutes. This treatment has no effect on Ub chain structure, but breaks soluble Ub chain aggregates.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.24% Tris, 0.87% Sodium chloride, 0.02% Beta mercaptoethanol, 10% Glycerol
General Info
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Alternative names
- UBB
- UBB_HUMAN
- Ubiquitin
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Function
Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. -
Sequence similarities
Belongs to the ubiquitin family.
Contains 3 ubiquitin-like domains. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
Images
References
ab206177 has not yet been referenced specifically in any publications.