Key features and details
- Expression system: HEK 293 cells
- Purity: > 98% SDS-PAGE
- Endotoxin level: < 0.100 Eu/µg
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE, ELISA
Product nameRecombinant human Polymeric immunoglobulin receptor/PIGR protein (Active)
See all Polymeric immunoglobulin receptor/PIGR proteins and peptides
Measured by its binding ability in a functional ELISA.
Immobilized ab174011 at 2 μg/ml, the concentration of Human IgM that produces 50% of the optimal binding response is appoximately 20-200 ng/ml.
Purity> 98 % SDS-PAGE.
ab174011 was lyophilized from 0.22 µm filtered solution.
Endotoxin level< 0.100 Eu/µg
Expression systemHEK 293 cells
Protein lengthProtein fragment
SequenceKSPIFGPEEVNSVEGNSVSITCYYPPTSVNRHTRKYWCRQGARGGCITLI SSEGYVSSKYAGRANLTNFPENGTFVVNIAQLSQDDSGRYKCGLGINSRG LSFDVSLEVSQGPGLLNDTKVYTVDLGRTVTINCPFKTENAQKRKSLYKQ IGLYPVLVIDSSGYVNPNYTGRIRLDIQGTGQLLFSVVINQLRLSDAGQY LCQAGDDSNSNKKNADLQVLKPEPELVYEDLRGSVTFHCALGPEVANVAK FLCRQSSGENCDVVVNTLGKRAPAFEGRILLNPQDKDGSFSVVITGLRKE DAGRYLCGAHSDGQLQEGSPIQAWQLFVNEESTIPRSPTVVKGVAGGSVA VLCPYNRKESKSIKYWCLWEGAQNGRCPLLVDSEGWVKAQYEGRLSLLEE PGNGTFTVILNQLTSRDAGFYWCLTNGDTLWRTTVEIKIIEGEPNLKVPG NVTAVLGETLKVPCHFPCKFSSYEKYWCKWNNTGCQALPSQDEGPSKAFV NCDENSRLVSLTLNLVTRADEGWYWCGVKQGHFYGETAAVYVAVEERKAA GSRDVSLAKADAAPDEKVLDSGFREIENKAIQDPRLFAEEKAVADTRDQA DGSRASVDSGSSEEQGGSSR
Predicted molecular weight69 kDa including tags
Amino acids19 to 638
TagsHis tag C-Terminus
Our Abpromise guarantee covers the use of ab174011 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Previously labelled as PIGR.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at 4°C (stable for up to 12 months). Store at -20°C long term. Avoid freeze / thaw cycle.
Constituent: 100% PBS
5-10% trehalose is commonly used for freeze drying, and after reconstitution, the trehalose is approximately about 3-5%.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionReconstitute with sterile deionized water to a concentration of 400 µg/ml.
- Hepatocellular carcinoma associated protein TB6
- Hepatocellular carcinoma-associated protein TB6
FunctionThis receptor binds polymeric IgA and IgM at the basolateral surface of epithelial cells. The complex is then transported across the cell to be secreted at the apical surface. During this process a cleavage occurs that separates the extracellular (known as the secretory component) from the transmembrane segment.
Sequence similaritiesContains 5 Ig-like V-type (immunoglobulin-like) domains.
modificationsN-glycosylation is not necessary for Ig binding.
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
Human pIgR (His Tag) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 98%.
The purity of ab174011 was determined by reduced SDS-PAGE and staining overnight with Coomassie Blue.
DTT-reduced Protein migrates as 85-95 kDa due to glycosylation.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab174011 has not yet been referenced specifically in any publications.