Product nameRecombinant human PP2A-alpha protein (Active)
See all PP2A-alpha proteins and peptides
Protein lengthFull length protein
SourceBaculovirus infected Sf9 cells
Amino Acid Sequence
SequenceMDEKVFTKEL DQWIEQLNEC KQLSESQVKS LCEKAKEILT KESNVQEVRC PVTVCGDVHG QFHDLMELFR IGGKSPDTNY LFMGDYVDRG YYSVETVTLL VALKVRYRER ITILRGNHES RQITQVYGFY DECLRKYGNA NVWKYFTDLF DYLPLTALVD GQIFCLHGGL SPSIDTLDHI RALDRLQEVP HEGPMCDLLW SDPDDRGGWG ISPRGAGYTF GQDISETFNH ANGLTLVSRA HQLVMEGYNW CHDRNVVTIF SAPNYCYRCG NQAAIMELDD TLKYSFLQFD PAPRRGEPHV TRRTPDYFL
Molecular weight59 kDa including tags
Amino acids1 to 309
TagsGST tag N-Terminus
Additional sequence information(NM 002715)
Our Abpromise guarantee covers the use of ab128557 in the following tested applications.
Purity> 70 % Densitometry.
For optimal storage, aliquot target into smaller quantities after centrifugation.
PP2A is a human tumor suppressor that inhibits cellular transformation.
ab128557 gene encodes a specific phosphotyrosyl phosphatase activator of the dimeric form of protein phosphatase-2A.
ab128557 holoenzyme is a heterotrimeric protein composed of a structural subunit A, a catalytic subunit C, and a regulatory subunit B.
ab128557 can alter the substrate specificity of PP2A leading to enhanced phosphotyrosine phosphatase activity and decreased phosphoserine phosphatase activity.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 25% Glycerol, 1.74% Sodium chloride, 0.42% MOPS
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- PP2A alpha
FunctionPP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53. Dephosphorylates SV40 large T antigen, preferentially on serine residues 120, 123, 677, and perhaps 679. The C subunit was most active, followed by the AC form, which was more active than the ABC form, and activity of all three forms was strongly stimulated by manganese, and to a lesser extent by magnesium. Dephosphorylation by the AC form, but not C or ABC form is inhibited by small T antigen.
Sequence similaritiesBelongs to the PPP phosphatase family. PP-1 subfamily.
modificationsReversibly methyl esterified on Leu-309. Carboxyl methylation may play a role in holoenzyme assembly. It varies during the cell cycle.
Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation.
Cellular localizationCytoplasm. Nucleus. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle pole. In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles. Centromeric localization requires the presence of SGOL2.
- Information by UniProt
Specific activity of ab128557: 208 nmol/min/mg (as per activity assay protocol).
Approximate MW: 59 kDa.
All lanes : Anti-PP2A alpha + beta antibody [EPR11787(B)] (ab168350) at 1/20000 dilution
Lane 1 :
Recombinant Human PPP2CB protein (ab128562)
Lane 2 : Recombinant human PP2A-alpha protein (ab128557)
Lysates/proteins at 0.015 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Performed under reducing conditions.
Observed band size: 59 kDa why is the actual band size different from the predicted?
Exposure time: 15 seconds
Blocking and dilution buffer: 5% NFDM/TBST.
ab128557 has not yet been referenced specifically in any publications.