Overview

Description

  • Nature

    Recombinant
  • Source

    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Accession
    • Species

      Human
    • Sequence

      MDEKVFTKEL DQWIEQLNEC KQLSESQVKS LCEKAKEILT KESNVQEVRC PVTVCGDVHG QFHDLMELFR IGGKSPDTNY LFMGDYVDRG YYSVETVTLL VALKVRYRER ITILRGNHES RQITQVYGFY DECLRKYGNA NVWKYFTDLF DYLPLTALVD GQIFCLHGGL SPSIDTLDHI RALDRLQEVP HEGPMCDLLW SDPDDRGGWG ISPRGAGYTF GQDISETFNH ANGLTLVSRA HQLVMEGYNW CHDRNVVTIF SAPNYCYRCG NQAAIMELDD TLKYSFLQFD PAPRRGEPHV TRRTPDYFL
    • Molecular weight

      59 kDa including tags
    • Amino acids

      1 to 309
    • Tags

      GST tag N-Terminus
    • Additional sequence information

      (NM 002715)

Specifications

Our Abpromise guarantee covers the use of ab128557 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    208 nmol/min/mg

  • Applications

    Western blot

    Phosphatase Activity

    SDS-PAGE

  • Purity

    > 70 % Densitometry.

  • Form

    Liquid
  • Additional notes

    For optimal storage, aliquot target into smaller quantities after centrifugation.

    PP2A is a human tumor suppressor that inhibits cellular transformation.

    ab128557 gene encodes a specific phosphotyrosyl phosphatase activator of the dimeric form of protein phosphatase-2A.

    ab128557 holoenzyme is a heterotrimeric protein composed of a structural subunit A, a catalytic subunit C, and a regulatory subunit B.

    ab128557 can alter the substrate specificity of PP2A leading to enhanced phosphotyrosine phosphatase activity and decreased phosphoserine phosphatase activity. 

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 25% Glycerol, 1.74% Sodium chloride, 0.42% MOPS

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • PP2A alpha
    • PP2A-alpha
    • PP2AA_HUMAN
    • PP2Aalpha
    • PP2Ac
    • PP2CA
    • PP2Calpha
    • PPP2CA
    • Protein phosphatase 2 (formerly 2A) catalytic subunit alpha isoform
    • Protein phosphatase 2 catalytic subunit alpha isoform
    • Protein phosphatase 2, catalytic subunit, alpha isozyme
    • Protein phosphatase 2A catalytic subunit, alpha isoform
    • Replication protein C
    • RP C
    • RP-C
    • RPC
    • Serine/threonine protein phosphatase 2A catalytic subunit alpha isoform
    • Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
    see all
  • Function

    PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53. Dephosphorylates SV40 large T antigen, preferentially on serine residues 120, 123, 677, and perhaps 679. The C subunit was most active, followed by the AC form, which was more active than the ABC form, and activity of all three forms was strongly stimulated by manganese, and to a lesser extent by magnesium. Dephosphorylation by the AC form, but not C or ABC form is inhibited by small T antigen.
  • Sequence similarities

    Belongs to the PPP phosphatase family. PP-1 subfamily.
  • Post-translational
    modifications

    Reversibly methyl esterified on Leu-309. Carboxyl methylation may play a role in holoenzyme assembly. It varies during the cell cycle.
    Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation.
  • Cellular localization

    Cytoplasm. Nucleus. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle pole. In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles. Centromeric localization requires the presence of SGOL2.
  • Information by UniProt

Images

  • Specific activity of ab128557: 208 nmol/min/mg (as per activity assay protocol).

  • Approximate MW: 59 kDa.

  • All lanes : Anti-PP2A alpha + beta antibody [EPR11787(B)] (ab168350) at 1/20000 dilution

    Lane 1 : Recombinant Human PPP2CB protein (ab128562)
    Lane 2 : Recombinant human PP2A-alpha protein (ab128557)

    Lysates/proteins at 0.015 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Performed under reducing conditions.

    Observed band size: 59 kDa
    why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    Blocking and dilution buffer: 5% NFDM/TBST.

References

ab128557 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for contacting us.

This product has been fully tested for its Phosphatase Activity and is fully guatranteed.

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