Recombinant human PPM1G protein (ab168041)

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SDS-PAGE - Recombinant human PPM1G protein (ab168041)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: >= 85% SDS-PAGE
    • Active: Yes
    • Tags: His tag N-Terminus
    • Suitable for: SDS-PAGE, Functional Studies

    Description

    • Product name

      Recombinant human PPM1G protein
      See all PPM1G proteins and peptides

    • Biological activity

      Specific activity: >5,000 units/mg. One unit will hydrolyze 1 nmole of pnitrophenylphosphatate (pNPP) per minute at 37°C, pH 7.5 using 10 mM of substrate.

    • Purity

      >= 85 % SDS-PAGE.

    • Expression system

      Escherichia coli

    • Accession

      O15355

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        MGSSHHHHHH SSGLVPRGSH MGAYLSQPNT VKCSGDGVGA PRLPLPYGFS AMQGWRVSME DAHNCIPELD SETAMFSVYD GHGGEEVALY CAKYLPDIIK DQKAYKEGKL QKALEDAFLA IDAKLTTEEV IKELAQIAGR PTEDEDEKEK VADEDDVDNE EAALLHEEAT MTIEELLTRY GQNCHKGPPH SKSGGGTGEE PGSQGLNGEA GPEDSTRETP SQENGPTAKA YTGFSSNSER GTEAGQVGEP GIPTGEAGPS CSSASDKLPR VAKSKFFEDS EDESDEAEEE EEDSEECSEE EDGYSSEEAE NEEDEDDTEE AEEDDEEEEE EMMVPGMEGK EEPGSDSGTT AVVALIRGKQ LIVANAGDSR CVVSEAGKAL DMSYDHKPED EVELARIKNA GGKVTMDGRV NGGLNLSRAI GDHFYKRNKN LPPEEQMISA LPDIKVLTLT DDHEFMVIAC DGIWNVMSSQ EVVDFIQSKI SQRDENGELR LLSSIVEELL DQCLAPDTSG DGTGCDNMTC IIICFKPRNT AELQPESGKR KLEEVLSTEG AEENGNSDKK KKAKRD

      • Predicted molecular weight

        61 kDa including tags

      • Amino acids

        1 to 546

      • Tags

        His tag N-Terminus

    Specifications

    Our Abpromise guarantee covers the use of ab168041 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      Functional Studies

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 8.00
      Constituents: 0.002% PMSF, 0.02% DTT, 0.32% Tris HCl, 20% Glycerol, 0.58% Sodium chloride

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    General Info

    • Alternative names

      • MGC1675
      • MGC2870
      • OTTHUMP00000123426
      • PP2C
      • PP2C gamma
      • PP2C-gamma
      • PP2CG
      • PP2Cgamma
      • PPM1G
      • PPM1G_HUMAN
      • PPP2CG
      • Protein phosphatase 1C
      • Protein phosphatase 1G
      • Protein phosphatase 2 catalytic subunit gamma isoform
      • Protein phosphatase 2C gamma isoform
      • Protein phosphatase 2C isoform gamma
      • Protein phosphatase magnesium dependent 1 gamma
      • Protein phosphatase magnesium-dependent 1 gamma
      • Protein phosphatase Mg2+/Mn2+ dependent 1G
      see all

    • Tissue specificity

      Widely expressed. Most abundant in testis, skeletal muscle, and heart.

    • Sequence similarities

      Belongs to the PP2C family.

    • Post-translational
      modifications

      Phosphorylated upon DNA damage, probably by ATM or ATR.

    • Cellular localization

      Cytoplasm.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant human PPM1G protein (ab168041)
      SDS-PAGE - Recombinant human PPM1G protein (ab168041)
      15% SDS-PAGE analysis of ab168041 (3µg).

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab168041? Please let us know so that we can cite the reference in this datasheet.

    ab168041 has not yet been referenced specifically in any publications.

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