Overview

  • Product name

    Recombinant Human PPP1R1A protein
  • Protein length

    Full length protein

Description

  • Nature

    Recombinant
  • Source

    Escherichia coli
  • Amino Acid Sequence
    • Species

      Human
    • Sequence

      MEQDNSPRKI QFTVPLLEPH LDPEAAEQIR RRRPTPATLV LTSDQSSPEI DEDRIPNPHL KSTLAMSPRQ RKKMTRITPT MKELQMMVEH HLGQQQQGEE PEGAAESTGT QESRPPGIPD TEVESRLGTS GTAKKTAECI PKTHERGSKE PSTKEPSTHI PPLDSKGANS VLEHHHHHH

Specifications

Our Abpromise guarantee covers the use of ab78756 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Purity

    > 90 % SDS-PAGE.
    ab78756 is purified by using anion-exchange chromatography (DEAE sepharose resin) and gel-filtration chromatography (Sephacryl S-200) with 20mM Tris pH 7.5, 2mM EDTA.
  • Form

    Liquid
  • Additional notes

    Previously labelled as Protein phosphatase inhibitor 1. 

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.00174% PMSF, 0.0154% DTT, 0.605% Tris, 0.0292% EDTA, 10% Glycerol

General Info

  • Alternative names

    • I 1
    • I-1
    • I1
    • Inhibitor 1
    • IPP 1
    • IPP-1
    • IPP1
    • Ppp1r1a
    • PPR1A_HUMAN
    • Protein phosphatase 1 regulatory (inhibitor) subunit 1A
    • Protein phosphatase 1 regulatory subunit 1A
    • Protein phosphatase inhibitor 1
    see all
  • Function

    Inhibitor of protein-phosphatase 1. This protein may be important in hormonal control of glycogen metabolism. Hormones that elevate intracellular cAMP increase I-1 activity in many tissues. I-1 activation may impose cAMP control over proteins that are not directly phosphorylated by PKA. Following a rise in intracellular calcium, I-1 is inactivated by calcineurin (or PP2B). Does not inhibit type-2 phosphatases.
  • Sequence similarities

    Belongs to the protein phosphatase inhibitor 1 family.
  • Post-translational
    modifications

    Phosphorylation of Thr-35 is required for activity.
  • Information by UniProt

Images

  • 15% SDS-PAGE showing ab78756 at approximately 20kDa (3µg).

References

ab78756 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for contacting us.

I am happy to help you with your inquiry.

Do you want to use these in your lysis buffer and keep the phosphorylation side of your target protein intact? If this is the case I am sorry to confirm that the two products you are asking for are not designed for a use like this. Unfortunatly we do currently not have any phosphatase inhibitors designed for this use.

If this is not the case could you please give me some further information to help me understand what exactly you need.

1. Could you briefly describe the whole experiment you would like do do?

2. Which antibodies exactly do you want to use in your WB application?

3. What samples are you going to use for your experiments?

Thank you in advance for your time. I hope that information will be helpful for you.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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