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    recombinant-human-ppp2cb-protein-ab128562.pdf

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Signal Transduction Protein Phosphorylation Ser / Thr Phosphatases
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Recombinant Human PPP2CB protein (ab128562)

  • Datasheet
  • SDS
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Western blot - Recombinant Human PPP2CB protein (ab128562)
  • SDS-PAGE - Recombinant Human PPP2CB protein (ab128562)

Key features and details

  • Expression system: Baculovirus infected Sf9 cells
  • Purity: > 75% Densitometry
  • Tags: GST tag N-Terminus
  • Suitable for: WB, SDS-PAGE

Description

  • Product name

    Recombinant Human PPP2CB protein
  • Purity

    > 75 % Densitometry.

  • Expression system

    Baculovirus infected Sf9 cells
  • Accession

    P62714
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Predicted molecular weight

      59 kDa including tags
    • Amino acids

      1 to 309
    • Tags

      GST tag N-Terminus

Associated products

  • Related Products

    • Anti-PPP2CB antibody (ab72343)

Specifications

Our Abpromise guarantee covers the use of ab128562 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    SDS-PAGE

  • Form

    Liquid
  • Additional notes

     This product was previously labelled as PP2A beta

     

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

General Info

  • Alternative names

    • PP2A beta
    • PP2A-beta
    • PP2AB_HUMAN
    • PP2Abeta
    • PP2CB
    • Ppp2cb
    • Protein phosphatase 2 (formerly 2A), catalytic subunit, beta isoform
    • Protein phosphatase 2 catalytic subunit beta isozyme
    • Protein phosphatase 2, catalytic subunit, beta isoform
    • Protein phosphatase 2A catalytic subunit beta isoform
    • Protein phosphatase type 2A catalytic subunit
    • Serine/threonine protein phosphatase 2A catalytic subunit beta
    • Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform
    see all
  • Function

    PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase.
  • Sequence similarities

    Belongs to the PPP phosphatase family. PP-1 subfamily.
  • Post-translational
    modifications

    Reversibly methyl esterified on Leu-309. Carboxyl methylation may play a role in holoenzyme assembly. It varies during the cell cycle. Demethylated by PME1 (in vitro).
    Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation.
  • Cellular localization

    Cytoplasm. Nucleus. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle pole. In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles.
  • Target information above from: UniProt accession P62714 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

Images

  • Western blot - Recombinant Human PPP2CB protein (ab128562)
    Western blot - Recombinant Human PPP2CB protein (ab128562)
    All lanes : Anti-PP2A alpha + beta antibody [EPR11787(B)] (ab168350) at 1/20000 dilution

    Lane 1 : Recombinant Human PPP2CB protein (ab128562)
    Lane 2 : Recombinant human PP2A-alpha protein (ab128557)

    Lysates/proteins at 0.015 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

    Observed band size: 59 kDa why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    Blocking and dilution buffer: 5% NFDM/TBST.

  • SDS-PAGE - Recombinant Human PPP2CB protein (ab128562)
    SDS-PAGE - Recombinant Human PPP2CB protein (ab128562)
    SDS-PAGE showing ab128562 at approximately 59 kDa

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab128562? Please let us know so that we can cite the reference in this datasheet.

ab128562 has not yet been referenced specifically in any publications.

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