Recombinant Human PPP2CB protein (ab128562)
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- References
- Protocols
Overview
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Product nameRecombinant Human PPP2CB protein
See all PPP2CB proteins and peptides -
Protein lengthFull length protein
Description
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NatureRecombinant
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SourceBaculovirus infected Sf9 cells
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Amino Acid Sequence
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Accession
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SpeciesHuman
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Molecular weight59 kDa including tags
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Amino acids1 to 309
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TagsGST tag N-Terminus
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab128562 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
SDS-PAGE
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Purity> 75 % Densitometry.
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FormLiquid
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Additional notes
This product was previously labelled as PP2A beta
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol, 0.88% Sodium chloride
General Info
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Alternative names
- PP2A beta
- PP2A-beta
- PP2AB_HUMAN
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FunctionPP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase.
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Sequence similaritiesBelongs to the PPP phosphatase family. PP-1 subfamily.
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Post-translational
modificationsReversibly methyl esterified on Leu-309. Carboxyl methylation may play a role in holoenzyme assembly. It varies during the cell cycle. Demethylated by PME1 (in vitro).
Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation. -
Cellular localizationCytoplasm. Nucleus. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle pole. In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles.
- Information by UniProt
Images
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Western blot - Recombinant Human PPP2CB protein (ab128562)All lanes : Anti-PP2A alpha + beta antibody [EPR11787(B)] (ab168350) at 1/20000 dilution
Lane 1 :Recombinant Human PPP2CB protein (ab128562)
Lane 2 : Recombinant human PP2A-alpha protein (ab128557)
Lysates/proteins at 0.015 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Observed band size: 59 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsBlocking and dilution buffer: 5% NFDM/TBST.
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SDS-PAGE - Recombinant Human PPP2CB protein (ab128562)SDS-PAGE showing ab128562 at approximately 59 kDa
Datasheets and documents
References
ab128562 has not yet been referenced specifically in any publications.
