Overview

Description

  • Nature
    Recombinant
  • Source
    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Molecular weight
      59 kDa including tags
    • Amino acids
      1 to 309
    • Tags
      GST tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab128562 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    SDS-PAGE

  • Purity
    > 75 % Densitometry.

  • Form
    Liquid
  • Additional notes

     This product was previously labelled as PP2A beta

     

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol, 0.88% Sodium chloride

General Info

  • Alternative names
    • PP2A beta
    • PP2A-beta
    • PP2AB_HUMAN
    • PP2Abeta
    • PP2CB
    • Ppp2cb
    • Protein phosphatase 2 (formerly 2A), catalytic subunit, beta isoform
    • Protein phosphatase 2 catalytic subunit beta isozyme
    • Protein phosphatase 2, catalytic subunit, beta isoform
    • Protein phosphatase 2A catalytic subunit beta isoform
    • Protein phosphatase type 2A catalytic subunit
    • Serine/threonine protein phosphatase 2A catalytic subunit beta
    • Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform
    see all
  • Function
    PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase.
  • Sequence similarities
    Belongs to the PPP phosphatase family. PP-1 subfamily.
  • Post-translational
    modifications
    Reversibly methyl esterified on Leu-309. Carboxyl methylation may play a role in holoenzyme assembly. It varies during the cell cycle. Demethylated by PME1 (in vitro).
    Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation.
  • Cellular localization
    Cytoplasm. Nucleus. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle pole. In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles.
  • Information by UniProt

Images

  • All lanes : Anti-PP2A alpha + beta antibody [EPR11787(B)] (ab168350) at 1/20000 dilution

    Lane 1 : Recombinant Human PPP2CB protein (ab128562)
    Lane 2 : Recombinant human PP2A-alpha protein (ab128557)

    Lysates/proteins at 0.015 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

    Observed band size: 59 kDa
    why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    Blocking and dilution buffer: 5% NFDM/TBST.

  • SDS-PAGE showing ab128562 at approximately 59 kDa

References

ab128562 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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