Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 80% Densitometry
- Active: Yes
- Suitable for: WB, Functional Studies, SDS-PAGE
Product nameRecombinant human PRAK/MK5 protein
See all PRAK/MK5 proteins and peptides
The specific activity of ab125743 was determined to be 172 nmol/min/mg.
Purity> 80 % Densitometry.
Purity was determined to be >80% by densitometry.
Expression systemBaculovirus infected Sf9 cells
Protein lengthFull length protein
Predicted molecular weight79 kDa including tags
Amino acids1 to 471
Our Abpromise guarantee covers the use of ab125743 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab204861 (Hsp27 peptide) can be utilized as a substrate for assessing kinase activity
This product was previously labelled as PRAK
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- MAP kinase-activated protein kinase 5
- MAPK-activated protein kinase 5
FunctionMediates stress-induced small heat shock protein 27 phosphorylation.
Tissue specificityExpressed ubiquitously.
Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
Contains 1 protein kinase domain.
modificationsPhosphorylated on Thr-182; which is the regulatory phosphorylation site and is located on the T-loop/loop 12.
Cellular localizationCytoplasm. Nucleus. Also observed in the nucleus.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab125743 has not yet been referenced specifically in any publications.