Recombinant Human PRAK/MK5 protein (ab217826)

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SDS-PAGE - Recombinant Human PRAK/MK5 protein (ab217826)

    Key features and details

    • Expression system: Baculovirus infected Sf9 cells
    • Purity: > 85% Densitometry
    • Tags: GST tag N-Terminus
    • Suitable for: WB, SDS-PAGE

    Description

    • Product name

      Recombinant Human PRAK/MK5 protein
      See all PRAK/MK5 proteins and peptides

    • Purity

      > 85 % Densitometry.

    • Expression system

      Baculovirus infected Sf9 cells

    • Accession

      Q8IW41

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        MSEESDMDKAIKETSILEEYSINWTQKLGAGISGPVRVCVKKSTQERFAL KILLDRPKARNEVRLHMMCATHPNIVQIIEVFANSVQFPHESSPRARLLI VMEMMEGGELFHRISQHRHFTEKQASQVTKQIALALRHCHLLNIAHRDLK PENLLFKDNSLDAPVKLCDFGFAKIDQGDLMTPQFTPYYVAPQVLEAQRR HQKEKSGIIPTSPTPYTYNKSCDLWSLGVIIYVMLCGYPPFYSKHHSRTI PKDMRRKIMTGSFEFPEEEWSQISEMAKDVVRKLLKVKPEERLTIEGVLD HPWLNSTEALDNVLPSAQLMMDKAVVAGIQQAHAEQLANMRIQDLKVSLK PLHSVNNPILRKRKLLGTKPKDSVYIHDHENGAEDSNVALEKLRDVIAQC ILPQAGKGENEDEKLNEVMQEAWKYNRECKLLRDTLQSFSWNGRGFTDKV DRLKLAEIVKQVIEEQTTSHESQ

      • Predicted molecular weight

        79 kDa

      • Amino acids

        1 to 473

      • Tags

        GST tag N-Terminus

      • Additional sequence information

        NM_003668.

    Specifications

    Our Abpromise guarantee covers the use of ab217826 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Western blot

      SDS-PAGE

    • Form

      Liquid

    • Additional notes

       This product was previously labelled as PRAK

       

    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

      pH: 7.50
      Constituents: 0.79% Tris HCl, 0.87% Sodium chloride, 0.31% Glutathione, 0.03% EDTA, 0.04% DTT, 0.002% PMSF, 25% Glycerol (glycerin, glycerine)

    General Info

    • Alternative names

      • MAP kinase-activated protein kinase 5
      • MAPK-activated protein kinase 5
      • MAPK5_HUMAN
      • MAPKAP kinase 5
      • MAPKAPK-5
      • MAPKAPK5
      • Mitogen activated protein kinase activated protein kinase 5
      • p38-regulated/activated protein kinase
      • PRAK
      see all

    • Function

      Mediates stress-induced small heat shock protein 27 phosphorylation.

    • Tissue specificity

      Expressed ubiquitously.

    • Sequence similarities

      Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
      Contains 1 protein kinase domain.

    • Post-translational
      modifications

      Phosphorylated on Thr-182; which is the regulatory phosphorylation site and is located on the T-loop/loop 12.

    • Cellular localization

      Cytoplasm. Nucleus. Also observed in the nucleus.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human PRAK/MK5 protein (ab217826)
      SDS-PAGE - Recombinant Human PRAK/MK5 protein (ab217826)

      SDS-PAGE analysis of ab217826.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab217826? Please let us know so that we can cite the reference in this datasheet.

    ab217826 has not yet been referenced specifically in any publications.

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