Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 68% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Product nameRecombinant human PRMT1 protein (Active)
See all PRMT1 proteins and peptides
Specific Activity: 6.2 pmol/min/µg.
Assay Conditions: 50 µl reaction mix (20 mM phosphate buffer pH 7.4, 20 µM S-adenosyl methionine, and 1-5 ng ab198122 add to the wells coated with the substrate. Incubate at room temperature for 1 hr. Add antibody against methylated R3 residue of histone H4, incubate 1 hr. Add secondary HRP-labeled antibody and incubate 30 min. Finally, add HRP chemiluminescent substrates and read luminesence.
Purity>= 68 % SDS-PAGE.
Expression systemBaculovirus infected Sf9 cells
Protein lengthFull length protein
SequenceAAAEAANCIMENFVATLANGMSLQPPLEEVSCGQAESSEKPNAEDMTSKD YYFDSYAHFGIHEEMLKDEVRTLTYRNSMFHNRHLFKDKVVLDVGSGTGI LCMFAAKAGARKVIGIECSSISDYAVKIVKANKLDHVVTIIKGKVEEVEL PVEKVDIIISEWMGYCLFYESMLNTVLYARDKWLAPDGLIFPDRATLYVT AIEDRQYKDYKIHWWENVYGFDMSCIKDVAIKEPLVDVVDPKQLVTNACL IKEVDIYTVKVEDLTFTSPFCLQVKRNDYVHALVAYFNIEFTRCHKRTGF STSPESPYTHWKQTVFYMEDYLTVKTGEEIFGTIGMRPNAKNNRDLDFTI DLDFKGQLCELSCSTDYRMR
Predicted molecular weight68 kDa including tags
Amino acids2 to 371
TagsGST tag N-Terminus
Our Abpromise guarantee covers the use of ab198122 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.63% Tris HCl, 1.74% Sodium chloride, 20% Glycerol, 0.02% DTT
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- ANM 1
FunctionArginine methyltransferase that methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in proteins such as ESR1, histone H2, H3 and H4, PIAS1, HNRNPA1, HNRNPD, NFATC2IP, SUPT5H, TAF15 and EWS. Constitutes the main enzyme that mediates monomethylation and asymmetric dimethylation of histone H4 'Arg-4' (H4R3me1 and H4R3me2a, respectively), a specific tag for epigenetic transcriptional activation. Together with dimethylated PIAS1, represses STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling. May be involved in the regulation of TAF15 transcriptional activity, act as an activator of estrogen receptor (ER)-mediated transactivation, play a key role in neurite outgrowth and act as a negative regulator of megakaryocytic differentiation, by modulating p38 MAPK pathway.
Tissue specificityWidely expressed.
Sequence similaritiesBelongs to the protein arginine N-methyltransferase family.
Cellular localizationNucleus. Cytoplasm > cytosol.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab198122 has not yet been referenced specifically in any publications.