Recombinant human PRMT3 protein (ab167953)

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Key features and details

  • Expression system: Baculovirus infected Sf9 cells
  • Purity: > 90% Densitometry
  • Active: Yes
  • Tags: proprietary tag N-Terminus
  • Suitable for: SDS-PAGE, WB, Functional Studies

Description

  • Product name

    Recombinant human PRMT3 protein

  • Biological activity

    The specific activity of ab167953 was determined to be 12 nmol/min/mg as per activity assay protocol.

  • Purity

    > 90 % Densitometry.

  • Expression system

    Baculovirus infected Sf9 cells

  • Accession

    Q8WUV3

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      MCSLASGATGGRGAVENEEDLPELSDSGDEAAWEDEDDADLPHGKQQTPC LFCNRLFTSAEETFSHCKSEHQFNIDSMVHKHGLEFYGYIKLINFIRLKN PTVEYMNSIYNPVPWEKEEYLKPVLEDDLLLQFDVEDLYEPVSVPFSYPN GLSENTSVVEKLKHMEARALSAEAALARAREDLQKMKQFAQDFVMHTDVR TCSSSTSVIADLQEDEDGVYFSSYGHYGIHEEMLKDKIRTESYRDFIYQN PHIFKDKVVLDVGCGTGILSMFAAKAGAKKVLGVDQSEILYQAMDIIRLN KLEDTITLIKGKIEEVHLPVEKVDVIISEWMGYFLLFESMLDSVLYAK NKYLAKGGSVYPDICTISLVAVSDVNKHADRIAFWDDVYGFKMSCMKKAV IPEAVVEVLDPKTLISEPCGIKHIDCHTTSISDLEFSSDFTLKITRTSMC TAIAGYFDIYFEKNCHNRVVFSTGPQSTKTHWKQTVFLLEKPFSVKAGEA LKGKVTVHKSKKDPRSLTVTLTLNNSTQTYGLQ

    • Predicted molecular weight

      97 kDa including tags

    • Amino acids

      1 to 531

    • Tags

      proprietary tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab167953 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Western blot

    Functional Studies

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.004% EDTA, 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • 2010005E20Rik
    • 2410018A17Rik
    • AL033309
    • ANM3_HUMAN
    • Heterogeneous nuclear ribonucleoprotein methyltransferase 1-like 3
    • Heterogeneous nuclear ribonucleoprotein methyltransferase like 3
    • Heterogeneous nuclear ribonucleoprotein methyltransferase like protein 3
    • Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 3
    • HMT1 hnRNP methyltransferase like 3
    • HMT1 hnRNP methyltransferase like 3 S. cerevisiae
    • HMT1-like 3
    • hnRNP methyltransferase like 3
    • HRMT1L3
    • PRMT 3
    • PRMT3
    • Protein arginine methyltransferase 3
    • Protein arginine N methyltransferase 3
    • Protein arginine N-methyltransferase 3
    see all

  • Function

    Methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in some proteins.

  • Sequence similarities

    Belongs to the protein arginine N-methyltransferase family.
    Contains 1 C2H2-type zinc finger.

  • Domain

    The zinc-finger is responsible for substrate specificity.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

Images

  • Functional Studies - Recombinant human PRMT3 protein (ab167953)
    Functional Studies - Recombinant human PRMT3 protein (ab167953)
    The specific activity of ab167953 was determined to be 12 nmol/min/mg as per activity assay protocol.
  • SDS-PAGE - Recombinant human PRMT3 protein (ab167953)
    SDS-PAGE - Recombinant human PRMT3 protein (ab167953)
    SDS-PAGE analysis of ab167953.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (1)

Publishing research using ab167953? Please let us know so that we can cite the reference in this datasheet.

ab167953 has been referenced in 1 publication.

  • Hsu MC  et al. Protein Arginine Methyltransferase 3 Enhances Chemoresistance in Pancreatic Cancer by Methylating hnRNPA1 to Increase ABCG2 Expression. Cancers (Basel) 11:N/A (2018). PubMed: 30577570

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