Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% Densitometry
- Active: Yes
- Tags: proprietary tag N-Terminus
- Suitable for: SDS-PAGE, WB, Functional Studies
Product nameRecombinant human PRMT3 protein
Biological activityThe specific activity of ab167953 was determined to be 12 nmol/min/mg as per activity assay protocol.
Purity> 90 % Densitometry.
Expression systemBaculovirus infected Sf9 cells
Protein lengthFull length protein
SequenceMCSLASGATGGRGAVENEEDLPELSDSGDEAAWEDEDDADLPHGKQQTPC LFCNRLFTSAEETFSHCKSEHQFNIDSMVHKHGLEFYGYIKLINFIRLKN PTVEYMNSIYNPVPWEKEEYLKPVLEDDLLLQFDVEDLYEPVSVPFSYPN GLSENTSVVEKLKHMEARALSAEAALARAREDLQKMKQFAQDFVMHTDVR TCSSSTSVIADLQEDEDGVYFSSYGHYGIHEEMLKDKIRTESYRDFIYQN PHIFKDKVVLDVGCGTGILSMFAAKAGAKKVLGVDQSEILYQAMDIIRLN KLEDTITLIKGKIEEVHLPVEKVDVIISEWMGYFLLFESMLDSVLYAK NKYLAKGGSVYPDICTISLVAVSDVNKHADRIAFWDDVYGFKMSCMKKAV IPEAVVEVLDPKTLISEPCGIKHIDCHTTSISDLEFSSDFTLKITRTSMC TAIAGYFDIYFEKNCHNRVVFSTGPQSTKTHWKQTVFLLEKPFSVKAGEA LKGKVTVHKSKKDPRSLTVTLTLNNSTQTYGLQ
Predicted molecular weight97 kDa including tags
Amino acids1 to 531
Tagsproprietary tag N-Terminus
Our Abpromise guarantee covers the use of ab167953 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.004% EDTA, 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
FunctionMethylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in some proteins.
Sequence similaritiesBelongs to the protein arginine N-methyltransferase family.
Contains 1 C2H2-type zinc finger.
DomainThe zinc-finger is responsible for substrate specificity.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab167953 has been referenced in 1 publication.
- Hsu MC et al. Protein Arginine Methyltransferase 3 Enhances Chemoresistance in Pancreatic Cancer by Methylating hnRNPA1 to Increase ABCG2 Expression. Cancers (Basel) 11:N/A (2018). PubMed: 30577570