Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 80% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Product nameRecombinant human PRMT8 protein (Active)
See all PRMT8 proteins and peptides
The specific activity of ab268892 was 35 nmol/min/mg in a methyltransferase assay using histone H4 peptide (1-21) as substrate.
Purity> 80 % SDS-PAGE.
Expression systemBaculovirus infected Sf9 cells
Protein lengthFull length protein
Molecular weight information7 kDa by SDS-PAGE
TagsGST tag N-Terminus
Additional sequence informationBC022458
Our Abpromise guarantee covers the use of ab268892 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.79% Tris HCl, 0.87% Sodium chloride, 0.31% Glutathione, 0.003% EDTA, 0.004% DTT, 0.002% PMSF, 25% Glycerol (glycerin, glycerine)
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- Heterogeneous nuclear ribonucleoprotein methyltransferase like protein 4
- Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 4
FunctionMembrane-associated arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and asymmetrical dimethylarginine (aDMA). Able to mono- and dimethylate EWS protein; however its precise role toward EWS remains unclear as it still interacts with fully methylated EWS.
Sequence similaritiesBelongs to the protein arginine N-methyltransferase family. PRMT8 subfamily.
DomainThe SH3-binding motifs mediate the interaction with SH3 domain-containing proteins such as PRMT2 and FYN, possibly leading to displace the N-terminal domain and activate the protein.
The N-terminal region (1-60) inhibits the enzymatic activity.
Cellular localizationCell membrane.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab268892 has not yet been referenced specifically in any publications.