Recombinant Human Procalcitonin protein (ab53369)

Description

  • Product name

    Recombinant Human Procalcitonin protein
    See all Procalcitonin proteins and peptides
  • Purity

    > 95 % SDS-PAGE.

  • Expression system

    Escherichia coli
  • Accession

  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Amino acids

      3 to 116
    • Tags

      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab53369 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.242% Tris, 50% Glycerol

General Info

  • Alternative names

    • CALC1
    • CALCA
    • calcitonin related polypeptide alpha
    • CGRP
    • CGRP1
    • CT
    • katacalcin
    • KC
    see all
  • Relevance

    Procalcitonin (PCT) is a 116 amino acid residue peptide with molecular weight of about 13 kDa. PCT itself has no known hormonal activity. PCT belongs to a group of related proteins including calcitonin gene-related peptides I and II, amylin, adrenomodulin and calcitonin (CAPA peptide family). PCT, like other peptides of CAPA family, appears from the common precursor pre-procalcitonin consisting of 141 amino acids by removal of 25 amino acids from the N-terminus. PCT undergoes successive cleavages to form three molecules: N-terminal fragment (55 a.a.), calcitonin (32 a.a.) and katacalcin (21 a.a.). Under normal metabolic conditions, PCT is only present in the C cells of the thyroid gland. In bacterial infection and sepsis, however, intact PCT is found in the blood and, more importantly, its level is related to the severity of bacterial sepsis. Today, PCT is considered to be one of the earliest and most specific markers of sepsis.
  • Cellular localization

    Secreted

Images

  • SDS-PAGE analysis of ab53369, amino-terminal hexahistidine tagged recombinant procalcitonin.

References

ab53369 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

All the information we have about an antibody we have you usually find on our datasheet. This includes tested species and application. According to the datasheet, WB hasn't been tested, and rat is only predicted to work but not tested. Therefore, we cannot send you any extra information.

However, I have attached an article in which is described what a good antibody is made of and you will see that all of the demands match this antibody.

To solve this problem in WB, I would suggest running a positive control in the form of the recombinant protein along your samples. If the signal is positive you can clearly show that the antibody detects the target. In addition, you could also use it instead of a blocking peptide in IHC along a isotype control to see if the specific staining will vanish. I have attached the protocol for this matter.



The protein can be found here:

https://www.abcam.com/index.html?datasheet=53369 (or use the following: https://www.abcam.com/index.html?datasheet=53369).



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