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    recombinant-human-procalcitonin-protein-ab92843.pdf

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Recombinant Human Procalcitonin protein (ab92843)

  • Datasheet
  • SDS
Submit a review Q&A (2)References (1)

Product price, shipping and contact information

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SDS-PAGE - Recombinant Human Procalcitonin protein (ab92843)
  • Western blot - Recombinant Human Procalcitonin protein (ab92843)
  • Western blot - Recombinant human Procalcitonin protein (ab92843)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 95% SDS-PAGE
  • Endotoxin level: < 1.000 Eu/µg
  • Suitable for: HPLC, SDS-PAGE, WB

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Description

  • Product name

    Recombinant Human Procalcitonin protein
    See all Procalcitonin proteins and peptides
  • Purity

    > 95 % SDS-PAGE.
    >95% as determined by RP-HPLC analyses. Purified using standard chromatographical techniques.
  • Endotoxin level

    < 1.000 Eu/µg
  • Expression system

    Escherichia coli
  • Accession

    P01258
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      APFRSALESS PADPATLSED EARLLLAALV QDYVQMKASE LEQEQEREGS SLDSPRSKRC GNLSTCMLGT YTQDFNKFHT FPQTAIGVGA PGKKRDMSSD LERDHRPHVS MPQNAN
    • Predicted molecular weight

      13 kDa
    • Amino acids

      26 to 141
    • Additional sequence information

      Mature protein without signal peptide.

Specifications

Our Abpromise guarantee covers the use of ab92843 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    HPLC

    SDS-PAGE

    Western blot

  • Form

    Lyophilized
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).

    Constituent: 0.16% Sodium phosphate

    Lyophilized from a sterile (0.2 micron) filtered aqueous solution.

  • Reconstitution
    Reconstitute at 0.1 mg/ml with sterile water. Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution.

General Info

  • Alternative names

    • CALC1
    • CALCA
    • calcitonin related polypeptide alpha
    • CGRP
    • CGRP1
    • CT
    • katacalcin
    • KC
    see all
  • Relevance

    Procalcitonin (PCT) is a 116 amino acid residue peptide with molecular weight of about 13 kDa. PCT itself has no known hormonal activity. PCT belongs to a group of related proteins including calcitonin gene-related peptides I and II, amylin, adrenomodulin and calcitonin (CAPA peptide family). PCT, like other peptides of CAPA family, appears from the common precursor pre-procalcitonin consisting of 141 amino acids by removal of 25 amino acids from the N-terminus. PCT undergoes successive cleavages to form three molecules: N-terminal fragment (55 a.a.), calcitonin (32 a.a.) and katacalcin (21 a.a.). Under normal metabolic conditions, PCT is only present in the C cells of the thyroid gland. In bacterial infection and sepsis, however, intact PCT is found in the blood and, more importantly, its level is related to the severity of bacterial sepsis. Today, PCT is considered to be one of the earliest and most specific markers of sepsis.
  • Cellular localization

    Secreted

Images

  • SDS-PAGE - Recombinant Human Procalcitonin protein (ab92843)
    SDS-PAGE - Recombinant Human Procalcitonin protein (ab92843)

    1ug of ab92843 was resolved on a 4-20% polyacrylamide, Tris-Glycine gel under non-reducing and reducing (+) conditions Recombinant Human Procalcitonin has a predicted molecular weight of 12.8kDa.

  • Western blot - Recombinant Human Procalcitonin protein (ab92843)
    Western blot - Recombinant Human Procalcitonin protein (ab92843)

    ab92843 used in Western Blot. Figure: 1 ug in each lane (-) non-reducing conditions and (+) reducing conditions in a 4-20% Tris-Glycine gel stained with Coomassie Blue.

    Human Procalcitonin has a predicted MW of 12.8 kDa.

  • Western blot - Recombinant human Procalcitonin protein (ab92843)
    Western blot - Recombinant human Procalcitonin protein (ab92843)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (1)

Publishing research using ab92843? Please let us know so that we can cite the reference in this datasheet.

ab92843 has been referenced in 1 publication.

  • Alshawawreh F  et al. The use of a personal glucose meter for detecting procalcitonin through glucose encapsulated within liposomes. Analyst 144:6225-6230 (2019). PubMed: 31555776

Customer reviews and Q&As

Show All Reviews Q&A
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1-2 of 2 Abreviews or Q&A

Question

Hello,

We have previously used this protein ab92843 Procalcitonin Protein (Active) in an immunomagnetic assay. I reordered the protein for continued use, and noticed that the signal in our assays has greatly increased with the use of the new order. The protein was resuspended and aliquoted following the same protocol as before.

When this order was shipped, it was delivered to a neighboring facility, and we were not informed. The package was not refrigerated, and the cold pack was no longer cold when I finally received the package. I expected this to cause a decrease in sensitivity/activity/signal, but found an increase, instead.

We have not experienced variability in Abcam products in the past. Could this variability have been caused by the improper warm storage of the product?

Read More

Abcam community

Verified customer

Asked on Jan 14 2013

Answer

Thank you for contacting us.
The shipment of the package should not have affected the activity of the antibody. Lyophilised protein is stable at room temperature for days. I am unsure as to why you have seen and increase in signal in this case, but please let me if the product is not working to your satisfaction.

Read More

Abcam Scientific Support

Answered on Jan 14 2013

Question

Good afternoon

Could you tell me if the following recombinant proteins possess any form of additional tag on the N or C terminus, please:

Ab92843 procalcitonin human recombinant protein

Ab50217 resistin human recombinant protein

Ab73252 resistin human recombinant protein


Kind regards

Read More

Abcam community

Verified customer

Asked on Jan 04 2013

Answer

Thank you for your enquiry. I appreciate your patience, it has regrettably taken some time to confirm the information for you.

I can let you know that these three proteins do not have a tag of any kind.

I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.

Read More

Abcam Scientific Support

Answered on Jan 04 2013

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