Recombinant Human proCathepsin D protein (ab151860)

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Key features and details

  • Expression system: HEK 293 cells
  • Purity: > 95% SDS-PAGE
  • Endotoxin level: < 1.000 Eu/µg
  • Tags: His tag C-Terminus
  • Suitable for: SDS-PAGE, HPLC

Description

  • Product name

    Recombinant Human proCathepsin D protein

  • Purity

    > 95 % SDS-PAGE.
    Purity is greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. ab151860 has been 0.2 µM filtered.

  • Endotoxin level

    < 1.000 Eu/µg

  • Expression system

    HEK 293 cells

  • Accession

    P07339

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      SALVRIPLHKFTSIRRTMSEVGGSVEDLIAKGPVSKYSQAVPAVTEGPIP EVLKNYMDAQYYGEIGIGTPPQCFTVVFDTGSSNLWVPSIHCKLLDIACW IHHKYNSDKSSTYVKNGTSFDIHYGSGSLSGYLSQDTVSVPCQSASSASA LGGVKVERQVFGEATKQPGITFIAAKFDGILGMAYPRISVNNVLPVFDNL MQQKLVDQNIFSFYLSRDPDAQPGGELMLGGTDSKYYKGSLSYLNVTRKA YWQVHLDQVEVASGLTLCKEGCEAIVDTGTSLMVGPVDEVRELQKAIGAV PLIQGEYMIPCEKVSTLPAITLKLGGKGYKLSPEDYTLKVSQAGKTLCLS GFMGMDIPPPSGPLWILGDVFIGRYYTVFDRDNNRVGFAEAARLVDHHHH HH

    • Predicted molecular weight

      44 kDa including tags

    • Amino acids

      19 to 412

    • Tags

      His tag C-Terminus

Specifications

Our Abpromise guarantee covers the use of ab151860 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    HPLC

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 5.50
    Constituents: 0.39% MES, 0.88% Sodium chloride

General Info

  • Alternative names

    • CATD_HUMAN
    • Cathepsin D
    • cathepsin D (lysosomal aspartyl protease)
    • Cathepsin D heavy chain
    • Ceroid-lipofuscinosis, neuronal 10
    • CLN10
    • CPSD
    • ctsd
    • EC 3.4.23
    • EC 3.4.23.5
    • Lysosomal aspartyl peptidase
    • Lysosomal aspartyl protease
    • MGC2311
    • OTTHUMP00000042282
    • OTTHUMP00000196039
    • OTTHUMP00000198692
    see all

  • Function

    Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.

  • Involvement in disease

    Defects in CTSD are the cause of neuronal ceroid lipofuscinosis type 10 (CLN10) [MIM:610127]; also known as neuronal ceroid lipofuscinosis due to cathepsin D deficiency. A form of neuronal ceroid lipofuscinosis with onset at birth or early childhood. Neuronal ceroid lipofuscinoses are progressive neurodegenerative, lysosomal storage diseases characterized by intracellular accumulation of autofluorescent liposomal material, and clinically by seizures, dementia, visual loss, and/or cerebral atrophy.

  • Sequence similarities

    Belongs to the peptidase A1 family.

  • Cellular localization

    Lysosome. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (1)

Publishing research using ab151860? Please let us know so that we can cite the reference in this datasheet.

ab151860 has been referenced in 1 publication.

  • Vangala G  et al. Mesenchymal stem cell homing towards cancer cells is increased by enzyme activity of cathepsin D. Exp Cell Res 383:111494 (2019). PubMed: 31306655

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