Recombinant Human Prohibitin protein (ab140551)

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SDS-PAGE - Recombinant Human Prohibitin protein (ab140551)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 85% SDS-PAGE
    • Tags: His tag N-Terminus
    • Suitable for: SDS-PAGE, MS

    Description

    • Product name

      Recombinant Human Prohibitin protein
      See all Prohibitin proteins and peptides

    • Purity

      > 85 % SDS-PAGE.
      ab140551 is purified using conventional chromatography techniques.

    • Expression system

      Escherichia coli

    • Accession

      P35232

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        MGSSHHHHHH SSGLVPRGSH MAAKVFESIG KFGLALAVAG GVVNSALYNV DAGHRAVIFD RFRGVQDIVV GEGTHFLIPW VQKPIIFDCR SRPRNVPVIT GSKDLQNVNI TLRILFRPVA SQLPRIFTSI GEDYDERVLP SITTEILKSV VARFDAGELI TQRELVSRQV SDDLTERAAT FGLILDDVSL THLTFGKEFT EAVEAKQVAQ QEAERARFVV EKAEQQKKAA IISAEGDSKA AELIANSLAT AGDGLIELRK LEAAEDIAYQ LSRSRNITYL PAGQSVLLQL PQ

      • Predicted molecular weight

        32 kDa including tags

      • Amino acids

        1 to 272

      • Tags

        His tag N-Terminus

    Specifications

    Our Abpromise guarantee covers the use of ab140551 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      Mass Spectrometry

    • Mass spectrometry

      MALDI-TOF

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 8.00
      Constituents: 0.32% Tris HCl, 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride

    General Info

    • Alternative names

      • Epididymis luminal protein 215
      • Epididymis secretory sperm binding protein Li 54e
      • HEL 215
      • HEL S 54e
      • PHB
      • PHB_HUMAN
      • PHB1
      • Prohibitin
      see all

    • Function

      Prohibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity and in aging.

    • Tissue specificity

      Widely expressed in different tissues.

    • Sequence similarities

      Belongs to the prohibitin family.

    • Developmental stage

      Levels of expression in fibroblasts decrease heterogeneously during cellular aging.

    • Cellular localization

      Mitochondrion inner membrane.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human Prohibitin protein (ab140551)
      SDS-PAGE - Recombinant Human Prohibitin protein (ab140551)

      15% SDS-PAGE analysis of ab140551 (3µg).

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab140551? Please let us know so that we can cite the reference in this datasheet.

    ab140551 has not yet been referenced specifically in any publications.

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