Recombinant Human Proteasome subunit beta type 2/PSMB2 protein (ab116169)

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SDS-PAGE - Recombinant Human Proteasome subunit beta type 2/PSMB2 protein (ab116169)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 90% SDS-PAGE
    • Tags: His tag N-Terminus
    • Suitable for: MS, SDS-PAGE

    Description

    • Product name

      Recombinant Human Proteasome subunit beta type 2/PSMB2 protein

    • Purity

      > 90 % SDS-PAGE.
      ab116169 is purified using conventional chromatography techniques.

    • Expression system

      Escherichia coli

    • Accession

      P49721

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        MGSSHHHHHHSSGLVPRGSHMEYLIGIQGPDYVLVASDRVAASNIVQMKD DHDKMFKMSEKILLLCVGEAGDTVQFAEYIQKNVQLYKMRNGYELSPTAA ANFTRRNLADCLRSRTPYHVNLLLAGYDEHEGPALYYMDY LAALAKAPFAAHGYGAFLTLSILDRYYTPTISRERAVELLRKCLEELQKR FILNLPTFSVRIIDKNGIHDLDNISFPKQGS

      • Predicted molecular weight

        25 kDa including tags

      • Amino acids

        1 to 201

      • Tags

        His tag N-Terminus

    Specifications

    Our Abpromise guarantee covers the use of ab116169 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Mass Spectrometry

      SDS-PAGE

    • Mass spectrometry

      MALDI-TOF

    • Form

      Liquid

    • Additional notes

       This product was previously labelled as PSMB2, Proteasome subunit beta type 2

       

    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 8.00
      Constituents: 0.02% DTT, 0.32% Tris HCl, 30% Glycerol (glycerin, glycerine), 0.58% Sodium chloride

    General Info

    • Alternative names

      • HC7 I
      • Macropain subunit C7 I
      • Macropain subunit C7-I
      • Multicatalytic endopeptidase complex subunit C7 1
      • Multicatalytic endopeptidase complex subunit C7 I
      • Multicatalytic endopeptidase complex subunit C7-I
      • Proteasome (prosome, macropain) subunit beta type 2
      • Proteasome beta 2 subunit
      • Proteasome component C7 I
      • Proteasome component C7-I
      • Proteasome subunit beta type-2
      • PSB2_HUMAN
      • Psmb2
      see all

    • Function

      The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit has a trypsin-like activity.

    • Sequence similarities

      Belongs to the peptidase T1B family.

    • Cellular localization

      Cytoplasm. Nucleus.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human Proteasome subunit beta type 2/PSMB2 protein (ab116169)
      SDS-PAGE - Recombinant Human Proteasome subunit beta type 2/PSMB2 protein (ab116169)
      15% SDS-PAGE showing ab116169 at approximately 24.9 kDa (3µg).

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab116169? Please let us know so that we can cite the reference in this datasheet.

    ab116169 has not yet been referenced specifically in any publications.

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