Recombinant Human Proteasome subunit beta type 2/PSMB2 protein (ab116169)

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Key features and details

  • Expression system: Escherichia coli
  • Purity: > 90% SDS-PAGE
  • Tags: His tag N-Terminus
  • Suitable for: MS, SDS-PAGE

Description

  • Product name

    Recombinant Human Proteasome subunit beta type 2/PSMB2 protein

  • Purity

    > 90 % SDS-PAGE.
    ab116169 is purified using conventional chromatography techniques.

  • Expression system

    Escherichia coli

  • Accession

    P49721

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      MGSSHHHHHHSSGLVPRGSHMEYLIGIQGPDYVLVASDRVAASNIVQMKD DHDKMFKMSEKILLLCVGEAGDTVQFAEYIQKNVQLYKMRNGYELSPTAA ANFTRRNLADCLRSRTPYHVNLLLAGYDEHEGPALYYMDY LAALAKAPFAAHGYGAFLTLSILDRYYTPTISRERAVELLRKCLEELQKR FILNLPTFSVRIIDKNGIHDLDNISFPKQGS

    • Predicted molecular weight

      25 kDa including tags

    • Amino acids

      1 to 201

    • Tags

      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab116169 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Mass Spectrometry

    SDS-PAGE

  • Mass spectrometry

    MALDI-TOF

  • Form

    Liquid

  • Additional notes

     This product was previously labelled as PSMB2, Proteasome subunit beta type 2

     

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.02% DTT, 0.32% Tris HCl, 30% Glycerol (glycerin, glycerine), 0.58% Sodium chloride

General Info

  • Alternative names

    • HC7 I
    • Macropain subunit C7 I
    • Macropain subunit C7-I
    • Multicatalytic endopeptidase complex subunit C7 1
    • Multicatalytic endopeptidase complex subunit C7 I
    • Multicatalytic endopeptidase complex subunit C7-I
    • Proteasome (prosome, macropain) subunit beta type 2
    • Proteasome beta 2 subunit
    • Proteasome component C7 I
    • Proteasome component C7-I
    • Proteasome subunit beta type-2
    • PSB2_HUMAN
    • Psmb2
    see all

  • Function

    The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit has a trypsin-like activity.

  • Sequence similarities

    Belongs to the peptidase T1B family.

  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant Human Proteasome subunit beta type 2/PSMB2 protein (ab116169)
    SDS-PAGE - Recombinant Human Proteasome subunit beta type 2/PSMB2 protein (ab116169)
    15% SDS-PAGE showing ab116169 at approximately 24.9 kDa (3µg).

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab116169? Please let us know so that we can cite the reference in this datasheet.

ab116169 has not yet been referenced specifically in any publications.

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