Recombinant Human PSIP1/LEDGF protein (ab82126)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Suitable for: SDS-PAGE
Description
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Product name
Recombinant Human PSIP1/LEDGF protein
See all PSIP1/LEDGF proteins and peptides -
Purity
> 95 % SDS-PAGE.
Purification by Ion Exchange Chromatography, purity determined by SDS-PAGE -
Expression system
Escherichia coli -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Additional sequence information
The amino acid sequence for ab82126 corresponds to isoform 2 of the protein, which is also known as p52 and PSIP2. Accession number NM_021144.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab82126 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Liquid -
Additional notes
This product was previously labelled as PSIP1.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.9
Constituents: 0.75% Potassium chloride, 0.0154% DTT, 0.316% Tris HCl, 0.00584% EDTA, 20% Glycerol (glycerin, glycerine)
General Info
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Alternative names
- CLL associated antigen KW 7
- CLL-associated antigen KW-7
- Dense fine speckles 70 kDa protein
see all -
Function
Transcriptional coactivator involved in neuroepithelial stem cell differentiation and neurogenesis. Involved in particular in lens epithelial cell gene regulation and stress responses. May play an important role in lens epithelial to fiber cell terminal differentiation. May play a protective role during stress-induced apoptosis. Isoform 2 is a more general and stronger transcriptional coactivator. Isoform 2 may also act as an adapter to coordinate pre-mRNA splicing. Cellular cofactor for lentiviral integration. -
Tissue specificity
Widely expressed. Expressed at high level in the thymus. Expressed in fetal and adult brain. Expressed in neurons, but not astrocytes. Markedly elevated in fetal as compared to adult brain. In the adult brain, expressed in the subventricular zone (SVZ), in hippocampus, and undetectable elsewhere. In the fetal brain, expressed in the germinal neuroepithelium and cortical plate regions. -
Involvement in disease
Note=A chromosomal aberration involving PSIP1 is associated with pediatric acute myeloid leukemia (AML) with intermediate characteristics between M2-M3 French-American-British (FAB) subtypes. Translocation t(9;11)(p22;p15) with NUP98. The chimeric transcript is an in-frame fusion of NUP98 exon 8 to PSIP1/LEDGF exon 4. -
Sequence similarities
Belongs to the HDGF family.
Contains 1 PWWP domain. -
Domain
Residues 340-417 are necessary and sufficient for the interaction with HIV-1 IN (IBD domain). -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. Remains chromatin-associated throughout the cell cycle. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab82126 has not yet been referenced specifically in any publications.