Overview

  • Product name

    Recombinant Human PSKH1 protein
  • Protein length

    Full length protein

Description

  • Nature

    Recombinant
  • Source

    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Accession
    • Species

      Human
    • Molecular weight

      72 kDa including tags
    • Amino acids

      2 to 424
    • Tags

      GST tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab131708 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Western blot

  • Purity

    > 70 % SDS-PAGE.
    Assessed by densitometry.
  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.31% Glutathione, 0.002% PMSF, 0.005% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol, 0.29% Sodium chloride

General Info

  • Alternative names

    • KPSH1_HUMAN
    • Protein serine kinase H1
    • PSK H1
    • PSK-H1
    • PSKH1
    • Serine/threonine protein kinase H1
    • Serine/threonine-protein kinase H1
    see all
  • Function

    May be a SFC-associated serine kinase (splicing factor compartment-associated serine kinase) with a role in intranuclear SR protein (non-snRNP splicing factors containing a serine/arginine-rich domain) trafficking and pre-mRNA processing.
  • Tissue specificity

    Expressed in all tissues and cell lines tested with the highest level of abundance in testis.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Autophosphorylated on serine residues.
    Myristoylated. Required for membrane association. Prerequisite for palmitoylation to occur.
    Palmitoylated.
  • Cellular localization

    Golgi apparatus. Cytoplasm > cytoskeleton > centrosome. Nucleus speckle. Endoplasmic reticulum membrane. Cell membrane. Cytoplasm. Localized in the brefeldin A-sensitive Golgi compartment, at centrosomes, in the nucleus with a somewhat speckle-like presence, membrane-associated to the endoplasmic reticulum (ER) and the plasma membrane (PM), and more diffusely in the cytoplasm. Found to concentrate in splicing factor compartments (SFCs) within the nucleus of interphase cells. The acylation-negative form may be only cytoplasmic and nuclear. Acylation seems to allow the sequestering to the intracellular membranes. Myristoylation may mediate targeting to the intracellular non-Golgi membranes and palmitoylation may mediate the targeting to the Golgi membranes. Dual acylation is required to stabilize the interaction with Golgi membranes.
  • Information by UniProt

Images

  • The purity of ab131708 was determined to be >70% by densitometry.

References

ab131708 has not yet been referenced specifically in any publications.

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