Overview

Description

  • Nature

    Recombinant
  • Source

    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species

      Human
    • Sequence

      MASLLKVDQE VKLKVDSFRE RITSEAEDLV ANFFPKKLLE LDSFLKEPIL NIHDLTQIHS DMNLPVPDPI LLTNSHDGLD GPTYKKRRLD ECEEAFQGTK VFVMPNGMLK SNQQLVDIIE KVKPEIRLLI EKCNTVKMWV QLLIPRIEDG NNFGVSIQEE TVAELRTVES EAASYLDQIS RYYITRAKLV SKIAKYPHVE DYRRTVTEID EKEYISLRLI ISELRNQYVT LHDMILKNIE KIKRPRSSNA ETLY
    • Molecular weight

      30 kDa
    • Amino acids

      1 to 267
    • Additional sequence information

      Isoform 2 (NM_176863)

Specifications

Our Abpromise guarantee covers the use of ab206156 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    ab206156 can stimulate the peptidase activities of the 20S proteasome.

  • Applications

    Functional Studies

    SDS-PAGE

  • Purity

    > 95 % SDS-PAGE.

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.

    Constituents: 0.24% Tris, 0.87% Sodium chloride, 0.02% Beta mercaptoethanol, 10% Glycerol

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • 11S regulator complex gamma subunit
    • 11S regulator complex subunit gamma
    • Activator of multicatalytic protease subunit 3
    • Ki
    • Ki antigen
    • Ki nuclear autoantigen
    • Ki, PA28 gamma
    • PA28 gamma
    • PA28g
    • PA28gamma
    • Proteasome (prosome, macropain) activator subunit 3
    • Proteasome (prosome, macropain) activator subunit 3 (PA28 gamma; Ki)
    • Proteasome activator 28 gamma
    • Proteasome activator 28 subunit gamma
    • Proteasome activator complex subunit 3
    • Proteasome activator subunit 3
    • PSME3
    • PSME3_HUMAN
    • REG GAMMA
    • REG-gamma
    see all
  • Function

    Subunit of the 11S REG-gamma (also called PA28-gamma) proteasome regulator, a donut-shaped homoheptamer which associates with the proteasome. 11S REG-gamma activates the trypsin-like catalytic subunit of the proteasome but inhibits the chymotrypsin-like and postglutamyl-preferring (PGPH) subunits. Facilitates the MDM2-p53/TP53 interaction which promotes ubiquitination- and MDM2-dependent proteasomal degradation of p53/TP53, limiting its accumulation and resulting in inhibited apoptosis after DNA damage. May also be involved in cell cycle regulation.
  • Sequence similarities

    Belongs to the PA28 family.
  • Domain

    The C-terminal sequences affect heptamer stability and proteasome affinity.
  • Post-translational
    modifications

    Phosphorylated by MAP3K3.
  • Cellular localization

    Nucleus. Cytoplasm. Localizes to the cytoplasm during mitosis following nuclear envelope breakdown at this distinct stage of the cell cycle which allows its interaction with MAP3K3 kinase.
  • Information by UniProt

Images

  • Activation of 5 nM 20S proteasome by 25 nM ab206156, the proteasome activity was assayed by using 50 µM Suc-LLVY-AMC as the substrate. The AMC fluorescence was monitored by a plate reader with excitation and emission filters of 360±40 nm and 460±30 nm, respectively.

  • Coomassie stained SDS PAGE of ab206156 (5 μg)

References

ab206156 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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