Description

  • Product name

    Recombinant Human PSME3 protein (His tag)
    See all PSME3 proteins and peptides
  • Purity

    > 95 % SDS-PAGE.

  • Endotoxin level

    < 1.000 Eu/µg
  • Expression system

    Escherichia coli
  • Accession

  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      MASLLKVDQE VKLKVDSFRE RITSEAEDLV ANFFPKKLLE LDSFLKEPIL NIHDLTQIHS DMNLPVPDPI LLTNSHDGLD GPTYKKRRLD ECEEAFQGTK VFVMPNGMLK SNQQLVDIIE KVKPEIRLLI EKCNTVKMWV QLLIPRIEDG NNFGVSIQEE TVAELRTVES EAASYLDQIS RYYITRAKLV SKIAKYPHVE DYRRTVTEID EKEYISLRLI ISELRNQYVT LHDMILKNIE KIKRPRSSNA ETLY
    • Predicted molecular weight

      30 kDa including tags
    • Amino acids

      1 to 254
    • Tags

      His tag N-Terminus
    • Additional sequence information

      Accession # NP_00578

Specifications

Our Abpromise guarantee covers the use of ab217811 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Form

    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -80°C.

    pH: 8.00
    Constituents: 5% Trehalose, 1.16% Sodium chloride, 0.61% Tris

    Lyophilized from 0.22 µm filtered solution.
    5-10% trehalose is commonly used for freeze drying, and after reconstitution, the trehalose is mostly about 3-5%

  • Reconstitution
    It is recommended to reconstitute the lyophilized product in 100 µL sterile deionized water to a final concentration of 1 mg/mL. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage. Storage: In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).

General Info

  • Alternative names

    • 11S regulator complex gamma subunit
    • 11S regulator complex subunit gamma
    • Activator of multicatalytic protease subunit 3
    • Ki
    • Ki antigen
    • Ki nuclear autoantigen
    • Ki, PA28 gamma
    • PA28 gamma
    • PA28g
    • PA28gamma
    • Proteasome (prosome, macropain) activator subunit 3
    • Proteasome (prosome, macropain) activator subunit 3 (PA28 gamma; Ki)
    • Proteasome activator 28 gamma
    • Proteasome activator 28 subunit gamma
    • Proteasome activator complex subunit 3
    • Proteasome activator subunit 3
    • PSME3
    • PSME3_HUMAN
    • REG GAMMA
    • REG-gamma
    see all
  • Function

    Subunit of the 11S REG-gamma (also called PA28-gamma) proteasome regulator, a donut-shaped homoheptamer which associates with the proteasome. 11S REG-gamma activates the trypsin-like catalytic subunit of the proteasome but inhibits the chymotrypsin-like and postglutamyl-preferring (PGPH) subunits. Facilitates the MDM2-p53/TP53 interaction which promotes ubiquitination- and MDM2-dependent proteasomal degradation of p53/TP53, limiting its accumulation and resulting in inhibited apoptosis after DNA damage. May also be involved in cell cycle regulation.
  • Sequence similarities

    Belongs to the PA28 family.
  • Domain

    The C-terminal sequences affect heptamer stability and proteasome affinity.
  • Post-translational
    modifications

    Phosphorylated by MAP3K3.
  • Cellular localization

    Nucleus. Cytoplasm. Localizes to the cytoplasm during mitosis following nuclear envelope breakdown at this distinct stage of the cell cycle which allows its interaction with MAP3K3 kinase.
  • Information by UniProt

Images

  • The purity of ab217811 was determined by DTT reduced SDS-PAGE and staining overnight with Coomassie Blue. Protein migrates as 28-33 kDa.

References

ab217811 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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