Bioactive grade

Recombinant human PSPN protein (Active) (Animal Free) (ab218098)

Description

  • Product name

    Recombinant human PSPN protein (Active) (Animal Free)
    See all PSPN proteins and peptides
  • Biological activity

    The activity is determined by a cell proliferation assay with TT Human medullary thyroid cancer cells and is typically 4-16 ng/mL. This corresponds to an expected specific activity of 2.5 x 105 units/mg.

  • Purity

    > 97 % SDS-PAGE.
    The purity of ab218098 is typically 97% as determined by reducing and non-reducing SDS-PAGE.
  • Endotoxin level

    < 0.050 Eu/µg
  • Expression system

    Escherichia coli
  • Accession

  • Protein length

    Protein fragment
  • Animal free

    Yes
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      MALSGPCQLW SLTLSVAELG LGYASEEKVI FRYCAGSCPR GARTQHGLAL ARLQGQGRAH GGPCCRPTRY TDVAFLDDRH RWQRLPQLSA AACGCGG
    • Predicted molecular weight

      10 kDa
    • Amino acids

      61 to 156
    • Additional sequence information

      Predicted MW: Dimer, 10.4/20.8 kDa (with 97/194 amino acids)
  • Description

    Recombinant human PSPN protein (Active)

Associated products

Specifications

Our Abpromise guarantee covers the use of ab218098 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

  • Form

    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at Room Temperature. Store at -20°C or -80°C.

    pH: 7.50
    Constituent: 0.16% Sodium phosphate

    Lyophilized from a sterile (0.2 micron) filtered aqueous solution.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Reconstitute in sterile water at 0.1 mg/mL. Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.

General Info

  • Alternative names

    • Persephin
    • PSP
    • PSPN
  • Relevance

    PSPN is a neurotrophic factor, belonging to the GDNF family. Neurotrophic factors are important for the proper development and maintenance of the nervous system. These factors promote neuronal survival and can prevent the neuronal degeneration associated with injury, toxin exposure, or neurodegenerative disease. PSPN has a similar structure to other family members, glial cell line-derived neurotrophic factor, Artemin and neurturin. All these proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. PSPN signals through a multicomponent receptor system, composed of RET and one of four GFR alpha (alpha1-alpha4) receptors. The GFRalpha4 was first identified in chicken and was later shown to be the preferential binding subunit for PSPN. In contrast to GDNF and Neurturin, PSPN does not support survival of peripheral neurons.
  • Cellular localization

    Secreted

Images

  • SDS-PAGE analysis of ab218098 (1μg) stained with Coomassie blue:

    Lane 1: non-reduced

    Lane 2: reduced

    4-20% Tris-Glycine gel

    Human PSPN is predicted to be a dimer (20.8 kDa)

References

ab218098 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab218098.
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