Product nameRecombinant human PTP1B protein (Active)
See all PTP1B proteins and peptides
Specific activity is > 10,000 units/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C.
- Prepare a 200ul reaction mix into a suitable container: The final concentrations are 10mM Tris(pH7.5), 50mM NaCl, 2mM DTT, 1mM MnCl2, 10mM pNPP.
- Pre-warm reaction mix in heat block at 37°C
- Add recombinant PTP1B solution with various concentrations (2ug, 3ug, 4ug) in 200ul reaction buffer.
- Mix by inversion and Incubate at 37°C for 10minutes.
- Stop the reaction by adding the 800ul of 0.1N NaOH.
- Record the absorbance at 405nm
Purity> 95 % SDS-PAGE.
This protein was purified by conventional column chromatographic techniques.
Expression systemEscherichia coli
Protein lengthFull length protein
SequenceMEMEKEFEQI DKSGSWAAIY QDIRHEASDF PCRVAKLPKN KNRNRYRDVS PFDHSRIKLH QEDNDYINAS LIKMEEAQRS YILTQGPLPN TCGHFWEMVW EQKSRGVVML NRVMEKGSLK CAQYWPQKEE KEMIFEDTNL KLTLISEDIK SYYTVRQLEL ENLTTQETRE ILHFHYTTWP DFGVPESPAS FLNFLFKVRE SGSLSPEHGP VVVHCSAGIG RSGTFCLADT CLLLMDKRKD PSSVDIKKVL LEMRKFRMGL IQTADQLRFS YLAVIEGAKF IMGDSSVQDQ WKELSHEDLE PPPEHIPPPP RPPKRILEPH N
Predicted molecular weight37 kDa
Amino acids1 to 321
Additional sequence information(NP_002818)
Our Abpromise guarantee covers the use of ab51277 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration determined by Bradford assay.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituents: 0.0156% Beta mercaptoethanol, 0.0154% DTT, 0.395% Tris HCl, 0.0292% EDTA, 20% Glycerol
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- Non receptor tyrosine phosphatase 1
- Protein phosphotyrosylphosphatase 1B
FunctionMay play an important role in CKII- and p60c-src-induced signal transduction cascades.
Sequence similaritiesBelongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily.
Contains 1 tyrosine-protein phosphatase domain.
modificationsOxidized on Cys-215; the Cys-SOH formed in response to redox signaling reacts with the alpha-amido of the following residue to form a 4-amino-3-isothiazolidinone serine cross-link, triggering a conformational change that inhibits substrate binding and activity. The active site can be restored by reduction.
Cellular localizationEndoplasmic reticulum membrane.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab51277 has not yet been referenced specifically in any publications.