Recombinant Human PU.1/Spi1 protein (denatured) (ab202185)
Key features and details
- Expression system: Escherichia coli
- Purity: > 85% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE
Description
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Product name
Recombinant Human PU.1/Spi1 protein (denatured)
See all PU.1/Spi1 proteins and peptides -
Purity
> 85 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MGSSHHHHHHSSGLVPRGSHMGSMLQACKMEGFPLVPPQPSEDLVPYDTD LYQRQTHEYYPYLSSDGESHSDHYWDFHPHHVHSEFESFAENNFTELQSV QPPQLQQLYRHMELEQMHVLDTPMVPPHPSLGHQVSYLPRMCLQYPSLSP AQPSSDEEEGERQSPPLEVSDGEADGLEPGPGLLPGETGSKKKIRLYQFL LDLLRSGDMKDSIWWVDKDKGTFQFSSKHKEALAHRWGIQKGNRKKMTYQ KMARALRNYGKTGEVKKVKKKLTYQFSGEVLGRGGLAERRHPPH -
Predicted molecular weight
34 kDa -
Amino acids
1 to 271 -
Tags
His tag N-Terminus -
Additional sequence information
NP_001074016.
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Description
Recombinant Human PU.1/Spi1 protein
Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab202185 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.32% Tris HCl, 10% Glycerol (glycerin, glycerine)
General Info
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Alternative names
- transcription factor spi1
- 31 kDa Transforming Protein
- 31 kDa-transforming protein
see all -
Function
Binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. Also binds RNA and may modulate pre-mRNA splicing. -
Sequence similarities
Belongs to the ETS family.
Contains 1 ETS DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
Images
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SDS-PAGE - Recombinant Human PU.1/Spi1 protein (denatured) (ab202185)
15% SDS-PAGE analysis of ab202185 (3 μg).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab202185 has not yet been referenced specifically in any publications.