Recombinant Human PU.1/Spi1 protein (denatured) (ab202185)

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Description

  • Product name

    Recombinant Human PU.1/Spi1 protein (denatured)
    See all PU.1/Spi1 proteins and peptides

  • Purity

    > 85 % SDS-PAGE.

  • Expression system

    Escherichia coli

  • Accession

    P17947-2

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      MGSSHHHHHHSSGLVPRGSHMGSMLQACKMEGFPLVPPQPSEDLVPYDTD LYQRQTHEYYPYLSSDGESHSDHYWDFHPHHVHSEFESFAENNFTELQSV QPPQLQQLYRHMELEQMHVLDTPMVPPHPSLGHQVSYLPRMCLQYPSLSP AQPSSDEEEGERQSPPLEVSDGEADGLEPGPGLLPGETGSKKKIRLYQFL LDLLRSGDMKDSIWWVDKDKGTFQFSSKHKEALAHRWGIQKGNRKKMTYQ KMARALRNYGKTGEVKKVKKKLTYQFSGEVLGRGGLAERRHPPH

    • Predicted molecular weight

      34 kDa

    • Amino acids

      1 to 271

    • Tags

      His tag N-Terminus

    • Additional sequence information

      NP_001074016.

  • Description

    Recombinant Human PU.1/Spi1 protein

Specifications

Our Abpromise guarantee covers the use of ab202185 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8
    Constituents: 0.32% Tris HCl, 10% Glycerol

General Info

  • Alternative names

    • transcription factor spi1
    • 31 kDa Transforming Protein
    • 31 kDa-transforming protein
    • cb1086
    • Hematopoietic transcription factor PU.1
    • OF
    • oncogene spi1
    • PU.1
    • SFFV virus-induced murine erythroleukemia oncogene, mouse, homolog of
    • SFPI1
    • si:by184l24.2
    • SPI 1
    • SPI 1 proto oncogene
    • SPI A
    • Spi1
    • SPI1_HUMAN
    • Spleen focus forming virus (SFFV) proviral integration oncogene spi1
    • Spleen focus forming virus proviral integration oncogene spi1
    • Transcription factor PU.1
    see all

  • Function

    Binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. Also binds RNA and may modulate pre-mRNA splicing.

  • Sequence similarities

    Belongs to the ETS family.
    Contains 1 ETS DNA-binding domain.

  • Cellular localization

    Nucleus.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant Human PU.1/Spi1 protein (denatured) (ab202185)
    SDS-PAGE - Recombinant Human PU.1/Spi1 protein (denatured) (ab202185)

    15% SDS-PAGE analysis of ab202185 (3 μg).

References

ab202185 has not yet been referenced specifically in any publications.

Publishing research using ab202185? Please let us know so that we can cite the reference in this datasheet.

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