Recombinant Human PU.1/Spi1 protein (His tag) (ab236335)

Submit a review Submit a question

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 85% SDS-PAGE
  • Tags: His tag N-Terminus
  • Suitable for: MS, SDS-PAGE

Description

  • Product name

    Recombinant Human PU.1/Spi1 protein (His tag)
    See all PU.1/Spi1 proteins and peptides

  • Purity

    > 85 % SDS-PAGE.

  • Expression system

    Escherichia coli

  • Accession

    P17947

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      MLQACKMEGFPLVPPPSEDLVPYDTDLYQRQTHEYYPYLSSDGESHSDHY WDFHPHHVHSEFESFAENNFTELQSVQPPQLQQLYRHMELEQMHVLDTPM VPPHPSLGHQVSYLPRMCLQYPSLSPAQPSSDEEEGERQSPPLEVSDGEA DGLEPGPGLLPGETGSKKKIRLYQFLLDLLRSGDMKDSIWWVDKDKGTFQ FSSKHKEALAHRWGIQKGNRKKMTYQKMARALRNYGKTGEVKKVKKKLTY QFSGEVLGRGGLAERRHPPH

    • Predicted molecular weight

      35 kDa including tags

    • Amino acids

      1 to 270

    • Tags

      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab236335 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Mass Spectrometry

    SDS-PAGE

  • Mass spectrometry

    LC-MS/MS

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)

General Info

  • Alternative names

    • transcription factor spi1
    • 31 kDa Transforming Protein
    • 31 kDa-transforming protein
    • cb1086
    • Hematopoietic transcription factor PU.1
    • OF
    • oncogene spi1
    • PU.1
    • SFFV virus-induced murine erythroleukemia oncogene, mouse, homolog of
    • SFPI1
    • si:by184l24.2
    • SPI 1
    • SPI 1 proto oncogene
    • SPI A
    • Spi1
    • SPI1_HUMAN
    • Spleen focus forming virus (SFFV) proviral integration oncogene spi1
    • Spleen focus forming virus proviral integration oncogene spi1
    • Transcription factor PU.1
    see all

  • Function

    Binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. Also binds RNA and may modulate pre-mRNA splicing.

  • Sequence similarities

    Belongs to the ETS family.
    Contains 1 ETS DNA-binding domain.

  • Cellular localization

    Nucleus.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant Human PU.1/Spi1 protein (His tag) (ab236335)
    SDS-PAGE - Recombinant Human PU.1/Spi1 protein (His tag) (ab236335)

    (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) ananlysis with 5% enrichment gel and 15% separation gel of ab236335.

  • Mass Spectrometry - Recombinant Human PU.1/Spi1 protein (His tag) (ab236335)
    Mass Spectrometry - Recombinant Human PU.1/Spi1 protein (His tag) (ab236335)

    Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of ab236335 could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) PU.1/Spi1.

  • Mass Spectrometry - Recombinant Human PU.1/Spi1 protein (His tag) (ab236335)
    Mass Spectrometry - Recombinant Human PU.1/Spi1 protein (His tag) (ab236335)

    Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of ab236335 could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) PU.1/Spi1.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab236335? Please let us know so that we can cite the reference in this datasheet.

ab236335 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

There are currently no Customer reviews or Questions for ab236335.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com