Recombinant Human PVRL1/NECTIN1 protein (Tagged) (ab271397)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 81% SDS-PAGE
- Tags: Avi tag C-Terminus, Fc tag C-Terminus
- Suitable for: Size Exclusion Chromatography, SDS-PAGE, WB
Description
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Product name
Recombinant Human PVRL1/NECTIN1 protein (Tagged)
See all PVRL1/NECTIN1 proteins and peptides -
Purity
>= 81 % SDS-PAGE. -
Expression system
HEK 293 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
QVVQVNDSMY GFIGTDVVLH CSFANPLPSV KITQVTWQKS TNGSKQNVAI YNPSMGVSVL APYRERVEFL RPSFTDGTIR LSRLELEDEG VYICEFATFP TGNRESQLNL TVMAKPTNWI EGTQAVLRAK KGQDDKVLVA TCTSANGKPP SVVSWETRLK GEAEYQEIRN PNGTVTVISR YRLVPSREAH QQSLACIVNY HMDRFKESLT LNVQYEPEVT IEGFDGNWYL QRMDVKLTCK ADANPPATEY HWTTLNGSLP KGVEAQNRTL FFKGPINYSL AGTYICEATN PIGTRSGQVE VNITEFPYTP SPPEHGRRAG PVPTA -
Predicted molecular weight
65 kDa including tags -
Molecular weight information
Runs at higher than predicted MW on SDS-PAGE due to glycosylation. -
Amino acids
31 to 355 -
Tags
Avi tag C-Terminus , Fc tag C-Terminus -
Additional sequence information
Avi-Tag fused to C-terminal of the Fc portion of Human IgG1. Genbank: NM_002855
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Specifications
Our Abpromise guarantee covers the use of ab271397 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Size Exclusion Chromatography
SDS-PAGE
Western blot
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.4
Constituents: 2.32% Sodium chloride, 0.02% Potassium chloride, 20% Glycerol (glycerin, glycerine), 0.13% Sodium phosphate
General Info
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Alternative names
- CD111
- CD111 antigen
- CLPED1
see all -
Function
Promotes cell-cell contacts by forming homophilic or heterophilic trans-dimers. Heterophilic interactions have been detected between PVRL1/nectin-1 and PVRL3/nectin-3 and between PVRL1/nectin-1 and PVRL4/nectin-4. -
Involvement in disease
Defects in PVRL1 are the cause of ectodermal dysplasia Margarita Island type (EDMI) [MIM:225060]; also known as Zlotogora-Ogur syndrome, cleft lip/palate-ectodermal dysplasia syndrome (CLPED1) or ectodermal dysplasia 4. Ectodermal dysplasia defines a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. EDMI is an autosomal recessive syndrome characterized by the association of cleft lip/palate, ectodermal dysplasia (sparse short and dry scalp hair, sparse eyebrows and eyelashes), and partial syndactyly of the fingers and/or toes. Two thirds of the patients do not manifest oral cleft but present with abnormal teeth and nails.
Defects in PVRL1 are the cause of non-syndromic orofacial cleft type 7 (OFC7) [MIM:225060]. Non-syndromic orofacial cleft is a common birth defect consisting of cleft lips with or without cleft palate. Cleft lips are associated with cleft palate in two-third of cases. A cleft lip can occur on one or both sides and range in severity from a simple notch in the upper lip to a complete opening in the lip extending into the floor of the nostril and involving the upper gum. -
Sequence similarities
Belongs to the nectin family.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab271397 has not yet been referenced specifically in any publications.