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    recombinant-human-pyk2-protein-ab60878.pdf

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Signal Transduction Protein Phosphorylation Tyrosine Kinases FAK / PYK
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Recombinant human PYK2 protein (ab60878)

  • Datasheet
  • SDS
Submit a review Q&A (1)

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Functional Studies - Recombinant human PYK2 protein (ab60878)
  • SDS-PAGE - Recombinant human PYK2 protein (ab60878)
  • SDS-PAGE - Recombinant human PYK2 protein (ab60878)
  • SDS-PAGE - Recombinant human PYK2 protein (ab60878)
  • Functional Studies - Recombinant human PYK2 protein (ab60878)

Key features and details

  • Expression system: Baculovirus infected Sf9 cells
  • Active: Yes
  • Suitable for: SDS-PAGE, Functional Studies

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Description

  • Product name

    Recombinant human PYK2 protein
    See all PYK2 proteins and peptides
  • Expression system

    Baculovirus infected Sf9 cells
  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Amino acids

      360 to 690

Associated products

  • Substrate reagent

    • Poly (Glu, Tyr) peptide (ab204877)

Specifications

Our Abpromise guarantee covers the use of ab60878 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

  • Form

    Liquid
  • Additional notes

    ab204877 (Poly (4:1 Glu, Tyr) peptide) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.00
    Preservative: 1.02% Imidazole
    Constituents: 0.00174% PMSF, 0.82% Sodium phosphate, 0.00308% DTT, 25% Glycerol (glycerin, glycerine), 1.74% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • CADTK
    • CAK-beta
    • CAKB
    • CAKbeta
    • Calcium regulated non receptor proline rich tyrosine kinase
    • Calcium-dependent tyrosine kinase
    • Cell adhesion kinase beta
    • E430023O05Rik
    • EC 2.7.10.2
    • FADK 2
    • FADK2
    • FAK2
    • FAK2_HUMAN
    • Focal adhesion kinase 2
    • MGC124628
    • PKB
    • Proline-rich tyrosine kinase 2
    • Protein kinase B
    • Protein Tyrosine Kinase 2 Beta
    • Protein-tyrosine kinase 2-beta
    • PTK
    • PTK2B
    • PTK2B protein tyrosine kinase 2 beta
    • PYK2
    • RAFTK
    • RAFTK2
    • Related adhesion focal tyrosine kinase
    see all
  • Function

    Involved in calcium induced regulation of ion channel and activation of the map kinase signaling pathway. May represent an important signaling intermediate between neuropeptide activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. Interacts with the SH2 domain of Grb2. May phosphorylate the voltage-gated potassium channel protein Kv1.2. Its activation is highly correlated with the stimulation of c-Jun N-terminal kinase activity. Involved in osmotic stress-dependent SNCA 'Tyr-125' phosphorylation. In concert with SRC, plays an important role in osteoclastic bone resorption. Both the formation of a SRC-PTK2B complex, and SRC kinase activity are necessary for this function. The Tyr-402 phosphorylated form serves as a docking site for SRC and is important for the organization of the osteoclast actin cytoskeleton and attachment sites and for bone resorption.
  • Tissue specificity

    Most abundant in the brain, with highest levels in amygdala and hippocampus. Low levels in kidney. Also expressed in spleen and lymphocytes.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.
    Contains 1 FERM domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Phosphorylated on tyrosine residues in response to various stimuli that elevate the intracellular calcium concentration, as well as by PKC activation. Recruitment by nephrocystin to cell matrix adhesions initiates Tyr-402 phosphorylation. In monocytes, adherence to substrata is required for tyrosine phosphorylation and kinase activation. Angiotensin II, thapsigargin and L-alpha-lysophosphatidic acid (LPA) also induce autophosphorylation and increase kinase activity.
  • Cellular localization

    Cytoplasm. Cell membrane. Interaction with nephrocystin induces the membrane-association of the kinase.
  • Target information above from: UniProt accession Q14289 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

Images

  • Functional Studies - Recombinant human PYK2 protein (ab60878)
    Functional Studies - Recombinant human PYK2 protein (ab60878)
    The specific activity of PYK2 (ab60878) was determined to be 216 nmol/min/mg as per activity assay protocol
  • SDS-PAGE - Recombinant human PYK2 protein (ab60878)
    SDS-PAGE - Recombinant human PYK2 protein (ab60878)
    SDS PAGE analysis of ab60878
  • SDS-PAGE - Recombinant human PYK2 protein (ab60878)
    SDS-PAGE - Recombinant human PYK2 protein (ab60878)
    SDS PAGE analysis of ab60878
  • SDS-PAGE - Recombinant human PYK2 protein (ab60878)
    SDS-PAGE - Recombinant human PYK2 protein (ab60878)
    ab60878 on SDS-PAGE, MW ~39 kDa.
  • Functional Studies - Recombinant human PYK2 protein (ab60878)
    Functional Studies - Recombinant human PYK2 protein (ab60878)
    Kinase activity plot

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab60878? Please let us know so that we can cite the reference in this datasheet.

    ab60878 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
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    Question

    Hi,   I have technical questions regarding 2 of your products: PYK2 protein (Active), cat. no ab60878, and PYK2 protein (His tag), cat. no ab42622.   I need to know the phosphorylation site of these 2 proteins. More specifically, I need to know if these proteins are phosphorylated at Tyrosine 402.  

    Read More

    Abcam community

    Verified customer

    Asked on May 18 2012

    Answer

    Thank you for contacting us. I do want to apologize for the tardiness of this response.

    I have been in contact with the originator labs for ab42622 and ab60878 PYK2 proteins, in regards to whether these products are phosphorylated at Y402. Unfortunately neither group was able to confirm whether Y402 is phosphorylated at this site and each recommended that phophorylation state may be determined by performing a western blot with a phospho specific antibody such as ab4800 (https://www.abcam.com/PYK2-phospho-Y402-antibody-ab4800.html).

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    Answered on May 18 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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