• Nature
  • Source
    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Species
    • Amino acids
      360 to 690

Associated products


Our Abpromise guarantee covers the use of ab60878 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications


    Functional Studies

  • Form
  • Additional notes

    ab204877 (Poly (4:1 Glu, Tyr) peptide) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.00
    Preservative: 1.02% Imidazole
    Constituents: 0.00174% PMSF, 0.82% Sodium phosphate, 0.00308% DTT, 25% Glycerol, 1.74% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • CADTK
    • CAK-beta
    • CAKB
    • CAKbeta
    • Calcium regulated non receptor proline rich tyrosine kinase
    • Calcium-dependent tyrosine kinase
    • Cell adhesion kinase beta
    • E430023O05Rik
    • EC
    • FADK 2
    • FADK2
    • FAK2
    • FAK2_HUMAN
    • Focal adhesion kinase 2
    • MGC124628
    • PKB
    • Proline-rich tyrosine kinase 2
    • Protein kinase B
    • Protein Tyrosine Kinase 2 Beta
    • Protein-tyrosine kinase 2-beta
    • PTK
    • PTK2B
    • PTK2B protein tyrosine kinase 2 beta
    • PYK2
    • RAFTK
    • RAFTK2
    • Related adhesion focal tyrosine kinase
    see all
  • Function
    Involved in calcium induced regulation of ion channel and activation of the map kinase signaling pathway. May represent an important signaling intermediate between neuropeptide activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. Interacts with the SH2 domain of Grb2. May phosphorylate the voltage-gated potassium channel protein Kv1.2. Its activation is highly correlated with the stimulation of c-Jun N-terminal kinase activity. Involved in osmotic stress-dependent SNCA 'Tyr-125' phosphorylation. In concert with SRC, plays an important role in osteoclastic bone resorption. Both the formation of a SRC-PTK2B complex, and SRC kinase activity are necessary for this function. The Tyr-402 phosphorylated form serves as a docking site for SRC and is important for the organization of the osteoclast actin cytoskeleton and attachment sites and for bone resorption.
  • Tissue specificity
    Most abundant in the brain, with highest levels in amygdala and hippocampus. Low levels in kidney. Also expressed in spleen and lymphocytes.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.
    Contains 1 FERM domain.
    Contains 1 protein kinase domain.
  • Post-translational
    Phosphorylated on tyrosine residues in response to various stimuli that elevate the intracellular calcium concentration, as well as by PKC activation. Recruitment by nephrocystin to cell matrix adhesions initiates Tyr-402 phosphorylation. In monocytes, adherence to substrata is required for tyrosine phosphorylation and kinase activation. Angiotensin II, thapsigargin and L-alpha-lysophosphatidic acid (LPA) also induce autophosphorylation and increase kinase activity.
  • Cellular localization
    Cytoplasm. Cell membrane. Interaction with nephrocystin induces the membrane-association of the kinase.
  • Information by UniProt


  • ab60878 on SDS-PAGE, MW ~39 kDa.
  • Kinase activity plot


ab60878 has not yet been referenced specifically in any publications.

Customer reviews and Q&As


Thank you for contacting us. I do want to apologize for the tardiness of this response.

I have been in contact with the originator labs for ab42622 and ab60878 PYK2 proteins, in regards to whether these products are phosphorylated at Y402. Unfortunately neither group was able to confirm whether Y402 is phosphorylated at this site and each recommended that phophorylation state may be determined by performing a western blot with a phospho specific antibody such as ab4800 (https://www.abcam.com/PYK2-phospho-Y402-antibody-ab4800.html).

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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