Recombinant Human QTRTD1 protein (ab187456)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, MS
Description
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Product name
Recombinant Human QTRTD1 protein -
Purity
> 95 % SDS-PAGE.
ab187456 was purified using conventional chromatography. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MGSSHHHHHHSSGLVPRGSHMGSMKLSLTKVVNGCRLGKIKNLGKTGDHT MDIPGCLLYTKTGSAPHLTHHTLHNIHGVPAMAQLTLSSLAEHHEVLTEY KEGVGKFIGMPESLLYCSLHDPVSPCPAGYVTNKSVSVWSVAGRVEMTVS KFMAIQKALQPDWFQCLSDGEVSCKEATSIKRVRKSVDRSLLFLDNCLRL QEESEVLQKSVIIGVIEGGDVMEERLRSARETAKRPVGGFLLDGFQGNPT TLEARLRLLSSVTAELPEDKPRLISGVSRPDEVLECIERGVDLFESFFPY QVTERGCALTFSFDYQPNPEETLLQQNGTQEEIKCMDQIKKIETTGCNQE ITSFEINLKEKKYQEDFNPLVRGCSCYCCKNHTRAYIHHLLVTNELLAGV LLMMHNFEHYFGFFHYIREALKSDKLAQLKELIHRQAS -
Predicted molecular weight
49 kDa including tags -
Amino acids
1 to 415 -
Tags
His tag N-Terminus
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab187456 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Mass Spectrometry
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Mass spectrometry
MALDI-TOF -
Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: 30% Glycerol (glycerin, glycerine), 0.03% DTT, 0.03% EDTA, 0.002% PMSF, 69% PBS
General Info
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Alternative names
- FLJ12960
- QTRD1_HUMAN
- qtrtd1
see all -
Function
Interacts with QTRT1 to form an active queuine tRNA-ribosyltransferase. This enzyme exchanges queuine for the guanine at the wobble position of tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), thereby forming the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine). -
Pathway
tRNA modification; tRNA-queuosine biosynthesis. -
Sequence similarities
Belongs to the queuine tRNA-ribosyltransferase family. QTRTD1 subfamily. -
Cellular localization
Cytoplasm. Mitochondrion. May associate with the mitochondrion outer membrane. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab187456 has not yet been referenced specifically in any publications.