Recombinant Human Quinone oxidoreductase protein (BSA and azide free) (ab180303)

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SDS-PAGE - Recombinant Human Quinone oxidoreductase protein (BSA and azide free) (ab180303)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 95% SDS-PAGE
    • Tags: His tag N-Terminus
    • Suitable for: MS, SDS-PAGE

    Description

    • Product name

      Recombinant Human Quinone oxidoreductase protein (BSA and azide free)

    • Purity

      > 95 % SDS-PAGE.
      ab180303 was purified by using conventional chromatography techniques.

    • Expression system

      Escherichia coli

    • Accession

      Q08257

    • Protein length

      Full length protein

    • Animal free

      No

    • Carrier free

      Yes

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        MGSSHHHHHHSSGLVPRGSHMGSMATGQKLMRAVRVFEFGGPEVLKLRSD IAVPIPKDHQVLIKVHACGVNPVETYIRSGTYSRKPLLPYTPGSDVAGVI EAVGDNASAFKKGDRVFTSSTISGGYAEYALAADHTVYKLPEKLDFKQGA AIGIPYFTAYRALIHSACVKAGESVLVHGSGGVGLAACQIARAYGLKILG TAGTEEGQKIVLQNGAHEVFNHREVNYIDKIKKYVGEKGIDIIIEMLANV NLSKDLSLLSHGGRVIVVGSRGTIEINPRDTMAKESSIIGVTLFSSTKEE FQQYAAALQAGMEIGWLKPVIGSQYPLEKVAEAHENIIHGSGATGKMILL L

      • Predicted molecular weight

        38 kDa including tags

      • Amino acids

        1 to 329

      • Tags

        His tag N-Terminus

      • Additional sequence information

        NP_001123514.

    Specifications

    Our Abpromise guarantee covers the use of ab180303 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Mass Spectrometry

      SDS-PAGE

    • Mass spectrometry

      MALDI-TOF

    • Form

      Liquid

    • Additional notes

       This product was previously labelled as CRYZ

       

    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 8.00
      Constituents: 0.32% Tris HCl, 0.88% Sodium chloride, 20% Glycerol (glycerin, glycerine), 0.02% DTT

    General Info

    • Alternative names

      • Crystallin zeta
      • Crystallin zeta (quinone reductase)
      • Cryz
      • DKFZp779E0834
      • FLJ41475
      • NADPH:quinone reductase
      • QOR_HUMAN
      • Quinone oxidoreductase
      • Zeta crystallin
      • Zeta-crystallin
      see all

    • Function

      Does not have alcohol dehydrogenase activity. Binds NADP and acts through a one-electron transfer process. Orthoquinones, such as 1,2-naphthoquinone or 9,10-phenanthrenequinone, are the best substrates (in vitro). May act in the detoxification of xenobiotics. Interacts with (AU)-rich elements (ARE) in the 3'-UTR of target mRNA species. Enhances the stability of mRNA coding for BCL2. NADPH binding interferes with mRNA binding.

    • Tissue specificity

      Only very low amounts in the lens.

    • Sequence similarities

      Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily.

    • Cellular localization

      Cytoplasm.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human Quinone oxidoreductase protein (BSA and azide free) (ab180303)
      SDS-PAGE - Recombinant Human Quinone oxidoreductase protein (BSA and azide free) (ab180303)

      15% SDS-PAGE analysis of ab180303 (3 µg).

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab180303? Please let us know so that we can cite the reference in this datasheet.

    ab180303 has not yet been referenced specifically in any publications.

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