Description

  • Product name

    Recombinant Human Rac1 protein
    See all Rac1 proteins and peptides
  • Purity

    > 95 % SDS-PAGE.
    ab89246 is purified using conventional chromatography techniques. Endotoxin: < 1.0 EU per 1 microgram of protein (determined by LAL method).
  • Expression system

    Escherichia coli
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human

Specifications

Our Abpromise guarantee covers the use of ab89246 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.0154% DTT, 0.316% Tris HCl, 0.0292% EDTA, 10% Glycerol

General Info

  • Alternative names

    • Cell migration inducing gene 5 protein
    • Cell migration-inducing gene 5 protein
    • MGC111543
    • MIG5
    • Migration inducing gene 5
    • Migration inducing protein 5
    • p21 Rac1
    • p21-Rac1
    • Rac 1
    • RAC1
    • RAC1_HUMAN
    • Ras like protein TC25
    • Ras related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1)
    • Ras-like protein TC25
    • Ras-related C3 botulinum toxin substrate 1
    • Rho family small GTP binding protein Rac1
    • TC 25
    • TC25
    see all
  • Function

    Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles. Rac1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophages (By similarity). Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly.
    Isoform B has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins. It is able to bind to the GTPase-binding domain of PAK but not full-length PAK in a GTP-dependent manner, suggesting that the insertion does not completely abolish effector interaction.
  • Tissue specificity

    Isoform B is predominantly identified in skin and epithelial tissues from the intestinal tract. Its expression is elevated in colorectal tumors at various stages of neoplastic progression, as compared to their respective adjacent tissues.
  • Sequence similarities

    Belongs to the small GTPase superfamily. Rho family.
  • Domain

    The effector region mediates interaction with DEF6.
  • Post-translational
    modifications

    AMPylation at Tyr-32 and Thr-35 are mediated by bacterial enzymes in case of infection by H.somnus and V.parahaemolyticus, respectively. AMPylation occurs in the effector region and leads to inactivation of the GTPase activity by preventing the interaction with downstream effectors, thereby inhibiting actin assembly in infected cells. It is unclear whether some human enzyme mediates AMPylation; FICD has such ability in vitro but additional experiments remain to be done to confirm results in vivo.
  • Cellular localization

    Cell membrane. Melanosome. Inner surface of plasma membrane possibly with attachment requiring prenylation of the C-terminal cysteine (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt

Images

  • 15% SDS-PAGE showing ab89246 at approximately 23.6kDa (3µg).

References

ab89246 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Vielen Dank für Ihre Anfrage. Gegen die genannten NADPH-Oxidase-Komponenten haben wir folgende Antikörper bzw. Proteine in unserem Katalog: Click here (or use the following: https://www.abcam.com/index.html?datasheet=109366). Click here (or use the following: https://www.abcam.com/index.html?datasheet=80508). Click here (or use the following: https://www.abcam.com/index.html?datasheet=33186). Click here (or use the following: https://www.abcam.com/index.html?datasheet=2244). Click here (or use the following: https://www.abcam.com/index.html?datasheet=118159). Click here (or use the following: https://www.abcam.com/index.html?datasheet=75941). Click here (or use the following: https://www.abcam.com/index.html?datasheet=115855). Click here (or use the following: https://www.abcam.com/index.html?datasheet=59355). Click here (or use the following: https://www.abcam.com/index.html?datasheet=111855). Click here (or use the following: https://www.abcam.com/index.html?datasheet=795). Click here (or use the following: https://www.abcam.com/index.html?datasheet=88031). Click here (or use the following: https://www.abcam.com/index.html?datasheet=98054). Click here (or use the following: https://www.abcam.com/index.html?datasheet=117065). Click here (or use the following: https://www.abcam.com/index.html?datasheet=114183). Click here (or use the following: https://www.abcam.com/index.html?datasheet=84657). Click here (or use the following: https://www.abcam.com/index.html?datasheet=40471). Click here (or use the following: https://www.abcam.com/index.html?datasheet=89246). Click here (or use the following: https://www.abcam.com/index.html?datasheet=82670). Click here (or use the following: https://www.abcam.com/index.html?datasheet=51014). Click here (or use the following: https://www.abcam.com/index.html?datasheet=84655). Click here (or use the following: https://www.abcam.com/index.html?datasheet=78684). Click here (or use the following: https://www.abcam.com/index.html?datasheet=87665). Vielleicht interessiert Sie in diesem Zusammenhang auch einer unserer Assaykits (NADP/NADPH): Click here (or use the following: https://www.abcam.com/index.html?datasheet=65349). Wir geben alle getesteten Spezies und Anwendungen auf unseren Produktdatenblättern an und aktualisieren diese sobald wir neue Informationen erhalten. Falls sich herausstellt, dass der Antikörper nicht so funktioniert, wie auf dem Datenblatt beschrieben und Sie sich innerhalb von sechs Monaten mit Ihrem Problem an uns wenden, werden wir Ihnen gerne Vorschläge zur Optimierung Ihres Protokolls oder gegebenenfalls einen Ersatz oder eine Gutschrift schicken. Falls der Antikörper in einer bisher nicht getesteten Anwendung oder Spezies verwendet wird (z.B. für ab111855 oder ab115855), qualifizieren Sie sich für unser Testprogramm: Bei diesem Angebot bekommen Sie einen 100%igen Rabatt für eine zukünftige Bestellung, wenn Sie uns ein Abreview mit dem Testresultat zusenden. Die Bedingungen zu unserem 100% Abreview Rabatt können Sie unter dem folgenden Link nachlesen: https://www.abcam.com/collaborationdiscount. Falls Sie daran interessiert sind oder weitere Fragen haben, kontaktieren Sie bitte unseren wissenschaftlichen Dienst. Die oben genannten Antikörper sind nur eine Auswahl und wir haben noch weitere gegen die entsprechenden Zielproteine erhältlich, die für unterschiedliche Anwendungen getestet und garantiert sind. Bei einer detaillierteren Suche bzw. Beratung bin ich Ihnen gerne weiter behilflich. Gerne senden wir Ihnen auch gerne ein Angebot zu, sobald Sie eine konkrete Auswahl getroffen haben. Ich hoffe, diese Informationen helfen Ihnen weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up