Product nameRecombinant human Rad51 protein (Active)
See all Rad51 proteins and peptides
Protein lengthFull length protein
Amino Acid Sequence
SequenceGSHMAMQMQL EANADTSVEE ESFGPQPISR LEQCGINAND VKKLEEAGFH TVEAVAYAPK KELINIKGIS EAKADKILAE AAKLVPMGFT TATEFHQRRS EIIQITTGSK ELDKLLQGGI ETGSITEMFG EFRTGKTQIC HTLAVTCQLP IDRGGGEGKA MYIDTEGTFR PERLLAVAER YGLSGSDVLD NVAYARAFNT DHQTQLLYQA SAMMVESRYA LLIVDSATAL YRTDYSGRGE LSARQMHLAR FLRMLLRLAD EFGVAVVITN QVVAQVDGAA MFAADPKKPI GGNIIAHAST TRLYLRKGRG ETRICKIYDS PCLPEAEAMF AINADGVGDA KD
Amino acids1 to 339
Our Abpromise guarantee covers the use of ab63808 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Retain nuclear filament forming and strand-exchange activity as well as interaction with Rad52.
ab63808 confirmed to possess single strand DNA stimulated ATPase activity.
Purity> 95 % SDS-PAGE.
Highly purified by several steps of chromatography.
This protein can be used for:
1) Studies on homologous recombination mechanism and SOS response.
2) Useful in the screening using probe from library by promotion of DNA hybridization.
3) Facilitate DNA observation by electron microscope due to nucleofilament formation with DNA.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituents: 0.75% Potassium chloride, 0.0154% DTT, 0.316% Tris HCl, 0.0146% EDTA, 10% Glycerol
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- BRCA1/BRCA2 containing complex, subunit 5
- BRCC 5
FunctionPlays an important role in homologous strand exchange, a key step in DNA repair through homologous recombination. Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. Binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange (PubMed:26681308). Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3.
Tissue specificityHighly expressed in testis and thymus, followed by small intestine, placenta, colon, pancreas and ovary. Weakly expressed in breast.
Involvement in diseaseBreast cancer
Mirror movements 2
Defects in RAD51 are found in a patient with microcephaly, mental retardation without bone marrow failure and pediatric cancers.
Sequence similaritiesBelongs to the RecA family. RAD51 subfamily.
Contains 1 HhH domain.
DomainThe nuclear localization may reside in the C-terminus (between 259 and 339 AA).
modificationsUbiquitinated by the SCF(FBXO18) E3 ubiquitin ligase complex, regulating RAD51 subcellular location and preventing its association with DNA.
Phosphorylated. Phosphorylation of Thr-309 by CHEK1 may enhance association with chromatin at sites of DNA damage and promote DNA repair by homologous recombination. Phosphorylation by ABL1 inhibits function.
Cellular localizationNucleus. Cytoplasm. Cytoplasm, perinuclear region. Mitochondrion matrix. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Colocalizes with RAD51AP1 and RPA2 to multiple nuclear foci upon induction of DNA damage. DNA damage induces an increase in nuclear levels. Together with FIGNL1, redistributed in discrete nuclear DNA damage-induced foci after ionizing radiation (IR) or camptothecin (CPT) treatment. Accumulated at sites of DNA damage in a SPIDR-dependent manner.
- Information by UniProt
ab63808 has not yet been referenced specifically in any publications.