Recombinant human Rad51 protein (Active) (ab63808)

Reviews (1)Q&A (2)References (1)
SDS-PAGE - Recombinant human Rad51 protein (ab63808)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 95% SDS-PAGE
    • Active: Yes
    • Suitable for: Functional Studies, SDS-PAGE, WB

    Description

    • Product name

      Recombinant human Rad51 protein (Active)
      See all Rad51 proteins and peptides

    • Biological activity

      Retain nuclear filament forming and strand-exchange activity as well as interaction with Rad52.

      ab63808 confirmed to possess single strand DNA stimulated ATPase activity.

    • Purity

      > 95 % SDS-PAGE.
      Highly purified by several steps of chromatography.

    • Expression system

      Escherichia coli

    • Accession

      Q06609

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        GSHMAMQMQL EANADTSVEE ESFGPQPISR LEQCGINAND VKKLEEAGFH TVEAVAYAPK KELINIKGIS EAKADKILAE AAKLVPMGFT TATEFHQRRS EIIQITTGSK ELDKLLQGGI ETGSITEMFG EFRTGKTQIC HTLAVTCQLP IDRGGGEGKA MYIDTEGTFR PERLLAVAER YGLSGSDVLD NVAYARAFNT DHQTQLLYQA SAMMVESRYA LLIVDSATAL YRTDYSGRGE LSARQMHLAR FLRMLLRLAD EFGVAVVITN QVVAQVDGAA MFAADPKKPI GGNIIAHAST TRLYLRKGRG ETRICKIYDS PCLPEAEAMF AINADGVGDA KD

      • Amino acids

        1 to 339

    Specifications

    Our Abpromise guarantee covers the use of ab63808 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Functional Studies

      SDS-PAGE

      Western blot

    • Form

      Liquid

    • Additional notes

      This protein can be used for:

      1) Studies on homologous recombination mechanism and SOS response.

      2) Useful in the screening using probe from library by promotion of DNA hybridization.

      3) Facilitate DNA observation by electron microscope due to nucleofilament formation with DNA.

    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

      pH: 6
      Constituents: 0.75% Potassium chloride, 0.0154% DTT, 0.316% Tris HCl, 0.0146% EDTA, 10% Glycerol (glycerin, glycerine)

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    General Info

    • Alternative names

      • BRCA1/BRCA2 containing complex, subunit 5
      • BRCC 5
      • BRCC5
      • DNA repair protein RAD51 homolog 1
      • DNA repair protein rhp51
      • FANCR
      • hRAD51
      • HsRAD51
      • HsT16930
      • MRMV2
      • Rad 51
      • RAD51
      • RAD51 homolog
      • RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae)
      • RAD51 homolog A
      • RAD51 recombinase
      • RAD51, S. cerevisiae, homolog of
      • RAD51_HUMAN
      • RAD51A
      • RECA
      • RecA like protein
      • RecA, E. coli, homolog of
      • Recombination protein A
      see all

    • Function

      Plays an important role in homologous strand exchange, a key step in DNA repair through homologous recombination. Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. Binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange (PubMed:26681308). Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3.

    • Tissue specificity

      Highly expressed in testis and thymus, followed by small intestine, placenta, colon, pancreas and ovary. Weakly expressed in breast.

    • Involvement in disease

      Breast cancer
      Mirror movements 2
      Defects in RAD51 are found in a patient with microcephaly, mental retardation without bone marrow failure and pediatric cancers.

    • Sequence similarities

      Belongs to the RecA family. RAD51 subfamily.
      Contains 1 HhH domain.

    • Domain

      The nuclear localization may reside in the C-terminus (between 259 and 339 AA).

    • Post-translational
      modifications

      Ubiquitinated by the SCF(FBXO18) E3 ubiquitin ligase complex, regulating RAD51 subcellular location and preventing its association with DNA.
      Phosphorylated. Phosphorylation of Thr-309 by CHEK1 may enhance association with chromatin at sites of DNA damage and promote DNA repair by homologous recombination. Phosphorylation by ABL1 inhibits function.

    • Cellular localization

      Nucleus. Cytoplasm. Cytoplasm, perinuclear region. Mitochondrion matrix. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Colocalizes with RAD51AP1 and RPA2 to multiple nuclear foci upon induction of DNA damage. DNA damage induces an increase in nuclear levels. Together with FIGNL1, redistributed in discrete nuclear DNA damage-induced foci after ionizing radiation (IR) or camptothecin (CPT) treatment. Accumulated at sites of DNA damage in a SPIDR-dependent manner.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant human Rad51 protein (ab63808)
      SDS-PAGE - Recombinant human Rad51 protein (ab63808)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (1)

    Publishing research using ab63808? Please let us know so that we can cite the reference in this datasheet.

    ab63808 has been referenced in 1 publication.

    • Daza-Martin M  et al. Isomerization of BRCA1-BARD1 promotes replication fork protection. Nature 571:521-527 (2019). PubMed: 31270457

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    Submitted Jan 25 2018

    Question

    I need the precise molecular weight of this recombinant protein please.

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    Answer

    This recombinant Rad51 is a full length protein (39,966 daltons according to Uniprot has additional peptide) which contains 3 additional amino acids, Gly-Ser-His, at the N-terminus.

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    Question

    Hi, Could you help us with the following enquiry? Rad51 protein (#AB63808). (Please note, it is not the antibody) Does this protein meet the following requirements? 1). The purity is better > 95% as judged from SDS-PAGE analysis 2). It should possess single strand DNA stimulated ATPase activity. 3). It can be used for the studies on homologous recombination in mammals including human 4). It can be used to study the interaction of Rad51 protein with various proteins. Thank you! Warmest Regards

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    Answer

    Thank you for your enquiry. I can confirm the following regarding ab63808 Rad51 protein: 1). The purity is better > 95% as judged from SDS-PAGE analysis Yes 2). It should possess single strand DNA stimulated ATPase activity. Yes 3). It can be used for the studies on homologous recombination in mammals including human Yes 4). It can be used to study the interaction of Rad51 protein with various proteins. Yes I hope this information will be helpful to you. Should you have any further questions, please do not hesitate to contact us.

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