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  1. Link

    recombinant-human-raf1-protein-ab90536.pdf

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Signal Transduction Protein Phosphorylation Ser / Thr Kinases Other Kinases
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Recombinant Human Raf1 protein (ab90536)

  • Datasheet
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Key features and details

  • Expression system: Escherichia coli
  • Purity: > 95% SDS-PAGE
  • Tags: GST tag N-Terminus
  • Suitable for: SDS-PAGE

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Description

  • Product name

    Recombinant Human Raf1 protein
    See all Raf1 proteins and peptides
  • Purity

    > 95 % SDS-PAGE.

  • Expression system

    Escherichia coli
  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Predicted molecular weight

      42 kDa including tags
    • Amino acids

      50 to 132
    • Tags

      GST tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab90536 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.60
    Constituents: 0.0475% Magnesium chloride, 0.5056% Tris HCl, 1.16% Sodium chloride

General Info

  • Alternative names

    • c Raf
    • C-raf
    • C-Raf proto-oncogene, serine/threonine kinase
    • CMD1NN
    • cRaf
    • Craf 1 transforming gene
    • Craf1 transforming gene
    • EC 2.7.11.1
    • kinase Raf1
    • Murine sarcoma 3611 oncogene 1
    • NS5
    • Oncogene MIL
    • Oncogene RAF1
    • OTTHUMP00000160218
    • OTTHUMP00000207813
    • OTTHUMP00000209389
    • Protein kinase raf 1
    • Proto-oncogene c-RAF
    • RAF
    • Raf 1
    • Raf 1 proto oncogene serine/threonine kinase
    • Raf proto oncogene serine/threonine protein kinase
    • RAF proto-oncogene serine/threonine-protein kinase
    • RAF-1
    • RAF1
    • RAF1_HUMAN
    • Similar to murine leukemia viral (V-raf-1) oncogene homolog 1
    • TRANSFORMING REPLICATION-DEFECTIVE MURINE RETROVIRUS 3611-MSV
    • v raf 1 murine leukemia viral oncogene homolog 1
    • v-raf murine sarcoma viral oncogene homolog 1
    • v-raf-1 murine leukemia viral oncogene-like protein 1
    • vraf1 murine leukemia viral oncogene homolog 1
    see all
  • Function

    Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3.
  • Tissue specificity

    In skeletal muscle, isoform 1 is more abundant than isoform 2.
  • Involvement in disease

    Defects in RAF1 are the cause of Noonan syndrome type 5 (NS5) [MIM:611553]. Noonan syndrome (NS) is a disorder characterized by dysmorphic facial features, short stature, hypertelorism, cardiac anomalies, deafness, motor delay, and a bleeding diathesis. It is a genetically heterogeneous and relatively common syndrome, with an estimated incidence of 1 in 1000-2500 live births.
    Defects in RAF1 are the cause of LEOPARD syndrome type 2 (LEOPARD2) [MIM:611554]. LEOPARD syndrome is an autosomal dominant disorder allelic with Noonan syndrome. The acronym LEOPARD stands for lentigines, electrocardiographic conduction abnormalities, ocular hypertelorism, pulmonic stenosis, abnormalities of genitalia, retardation of growth, and deafness.
  • Sequence similarities

    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.
    Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 protein kinase domain.
    Contains 1 RBD (Ras-binding) domain.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylation at Thr-269 increases its kinase activity. Phosphorylation at Ser-259 induces the interaction with YWHAZ and inactivates kinase activity. Dephosphorylation of Ser-259 by the complex containing protein phosphatase 1, SHOC2 and M-Ras/MRAS relieves inactivation, leading to stimulate RAF1 activity.
  • Cellular localization

    Cytoplasm. Cell membrane. Colocalizes with RGS14 and BRAF in both the cytoplasm and membranes.
  • Target information above from: UniProt accession P04049 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (0)

Publishing research using ab90536? Please let us know so that we can cite the reference in this datasheet.

ab90536 has not yet been referenced specifically in any publications.

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