Recombinant human RAGE protein (Fc Chimera Active) (ab219875)
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Overview
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Product nameRecombinant human RAGE protein (Fc Chimera Active)
See all RAGE proteins and peptides -
Protein lengthProtein fragment
Description
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NatureRecombinant
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SourceHEK 293 cells
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Amino Acid Sequence
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Accession
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SpeciesHuman
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SequenceAQNITARIGEPLVLKCKGAPKKPPQRLEWKLNTGRTEAWKVLSPQGGGPW DSVARVLPNGSLFLPAVGIQDEGIFRCQAMNRNGKETKSNYRVRVYQIPG KPEIVDSASELTAGVPNKVGTCVSEGSYPAGTLSWHLDGKPLVPNEKGVS VKEQTRRHPETGLFTLQSELMVTPARGGDPRPTFSCSFSPGLPRHRALRT APIQPRVWEPVPLEEVQLVVEPEGGAVAPGGTVTLTCEVPAQPSPQIHWM KDGVPLPLPPSPVLILPEIGPQDQGTYSCVATHSSHGPQESRAVSISIIE PGEEGPTAGSVGGSGLGTLALA
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Molecular weight61 kDa
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Amino acids23 to 344
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Additional sequence informationExtracellular domain with a human IgG1 Fc tag at the C-terminus.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab219875 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Biological activity
Immobilized ab219875 at 20 μg/mL (100 μL/well) can bind Human HMGB1, His Tag with a linear range of 0.313-5 μg/mL.
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Applications
SDS-PAGE
Functional Studies
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Endotoxin level< 1.000 Eu/µg
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Purity> 60 % SDS-PAGE.
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FormLyophilised
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.50
Constituents: 0.75% Glycine, 5% Trehalose, 0.61% TrisThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionIt is recommended to reconstitute the lyophilized product in sterile deionized water to a final concentration of 1 mg/mL. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage. Storage: In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).
General Info
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Alternative names
- Advanced glycosylation end product-specific receptor
- Ager
- MGC2235
see all -
FunctionMediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling (By similarity). Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space.
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Tissue specificityEndothelial cells.
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Sequence similaritiesContains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
Images
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SDS-PAGE - Recombinant human RAGE protein (Fc Chimera Active) (ab219875)ab219875 on SDS-PAGE under reducing (R) conditions. The gel was stained overnight with Coomassie Blue. The protein migrates as 80-90 kDa and 35 kDa Fc fragment under reducing (R) conditions due to glycosylation and auto-cleavage.
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Functional Studies - Recombinant human RAGE protein (Fc Chimera Active) (ab219875)Immobilized ab219875 at 20 μg/mL (100 μL/well) can bind Human HMGB1, His Tag with a linear range of 0.313-5 μg/mL.
Datasheets and documents
References
ab219875 has not yet been referenced specifically in any publications.
