Recombinant Human Ran (mutated E70A) protein (ab90203)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Suitable for: SDS-PAGE
Description
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Product name
Recombinant Human Ran (mutated E70A) protein
See all Ran proteins and peptides -
Biological activity
Protein preparation is 85% GDP-loaded, measured by HPLC. -
Purity
> 95 % SDS-PAGE. -
Expression system
Escherichia coli -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Modifications
mutated E70A
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab90203 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Liquid -
Additional notes
The mutation E70A results in inhibition of the guanine exchange reaction mediated by RCC1, the guanine nucleotide exchange factor (GEF) for Ran.
Protein preparation is 85% GDP-loaded, measured by HPLC. -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.40
Constituents: 0.0154% DTE (1,4-Dithioerythritol), 0.42% Potassium phosphate, 0.0475% Magnesium chloride
General Info
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Alternative names
- Androgen receptor associated protein 24
- Androgen receptor-associated protein 24
- ARA 24
see all -
Function
GTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export. Involved in chromatin condensation and control of cell cycle (By similarity). The complex with BIRC5/ survivin plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules.
Enhances AR-mediated transactivation. Transactivation decreases as the poly-Gln length within AR increases. -
Tissue specificity
Expressed in a variety of tissues. -
Sequence similarities
Belongs to the small GTPase superfamily. Ran family. -
Post-translational
modificationsThe N-terminus is blocked. -
Cellular localization
Nucleus. Cytoplasm. Melanosome. Becomes dispersed throughout the cytoplasm during mitosis. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (1)
ab90203 has been referenced in 1 publication.
- Fasci D et al. Histone Interaction Landscapes Visualized by Crosslinking Mass Spectrometry in Intact Cell Nuclei. Mol Cell Proteomics 17:2018-2033 (2018). PubMed: 30021884