Recombinant Human RanBP3 protein (ab181938)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: MS, SDS-PAGE
Description
-
Product name
Recombinant Human RanBP3 protein -
Purity
> 95 % SDS-PAGE.
ab181938 was purified using conventional chromatography techniques. -
Expression system
Escherichia coli -
Accession
-
Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
-
Species
Human -
Sequence
MGSSHHHHHHSSGLVPRGSHMGSISSSLENSTNSADASSNKFVFGQNMSE RVLSPPKLNEVSSDANRENAAAESGSESSSQEATPEKESLAESAAAYTKA TARKCLLEKVEVITGEEAESNVLQMQCKLFVFDKTSQSWVERGRGLLRLN DMASTDDGTLQSRLVMRTQGSLRLILNTKLWAQMQIDKASEKSIRITAMD TEDQGVKVFLISASSKDTGQLYAALHHRILALRS -
Predicted molecular weight
26 kDa including tags -
Amino acids
235 to 445 -
Tags
His tag N-Terminus -
Additional sequence information
NP_015559
-
Associated products
-
Related Products
Specifications
Our Abpromise guarantee covers the use of ab181938 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
Applications
Mass Spectrometry
SDS-PAGE
-
Mass spectrometry
MALDI-TOF -
Form
Liquid -
Concentration information loading...
Preparation and Storage
-
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 10% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl
General Info
-
Alternative names
- DKFZp586I1520
- Ran binding protein 3
- Ran-binding protein 3
see all -
Function
Acts as a cofactor for XPO1/CRM1-mediated nuclear export, perhaps as export complex scaffolding protein. Bound to XPO1/CRM1, stabilizes the XPO1/CRM1-cargo interaction. In the absence of Ran-bound GTP prevents binding of XPO1/CRM1 to the nuclear pore complex. Binds to CHC1/RCC1 and increases the guanine nucleotide exchange activity of CHC1/RCC1. Recruits XPO1/CRM1 to CHC1/RCC1 in a Ran-dependent manner. Negative regulator of TGF-beta signaling through interaction with the R-SMAD proteins, SMAD2 and SMAD3, and mediating their nuclear export. -
Tissue specificity
Widely expressed with high levels in testis and heart. -
Sequence similarities
Contains 1 RanBD1 domain. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab181938 has not yet been referenced specifically in any publications.